Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Lane 3: Human U251 cell lysates
Primary: Anti- Orexin A (bs-15509R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13 kDa
Observed band size: 12 kDa
- ￥1180 - 1980
- Bioss
- bs-15509R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 产品编号 | bs-15509R |
| 英文名称 | Orexin A Rabbit pAb |
| 中文名称 | 增食欲素A/欲激素A抗体 |
| 英文别名 | orexin-A; HCRT 1; HCRT; HCRT1; Hypocretin(orexin) neuropeptide precursor; Hypocretin 1; Hypocretin A; Hypocretin; Hypocretin neuropeptide precursor; Hypocretin1; Orexin A; Orexin; Orexin precursor; OrexinA; OX; PPORX; PPOX; PREPROOREXIN; ORX-A; OREX_HUMAN. |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Orexin A |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 13 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Metabolism > Types of disease > Obesity Neuroscience > Neurology process > Metabolism Neuroscience > Neurotransmitter > Neuropeptides > Hormones |
| 亚细胞定位 | Cytoplasmic and Endoplasmic reticulum |
| 组织特异性 | Abundantly expressed in subthalamic nucleus but undetectable in other brain regions tested (hypothalamus was not tested) and in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas. |
| 翻译后修饰 | Specific enzymatic cleavages at paired basic residues yield the different active peptides. |
| 相似性 | Belongs to the orexin family. |
| 功能 | Prepro-orexin is 130 amino acid long peptide with a putative 33 AA secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis. These neuropeptides bind and activate two closely related Orexin receptors—G-protein coupled receptors (GPCRs) OX1R and OX2R. Orexins (Orexin A and Orexin B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus. Orexin A and Orexin B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Prepro-orexin is 130 amino acid long peptide with a putative 33 AA secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis. These neuropeptides bind and activate two closely related Orexin receptors—G-protein coupled receptors (GPCRs) OX1R and OX2R. Orexins (Orexin A and Orexin B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus. Orexin A and Orexin B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {ELISA} | {1:5000-10000} |
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文献和实验该产品被引用文献
[IF={{ 9.3 }}] {Guo Jing. et al. Therapeutic effects of orexin-A in sepsis-associated encephalopathy in mice. J NEUROINFLAMM. 2024 Dec;21(1):1-19} {WB} {Mouse}
[IF={{ 2.751 }}] {De-Qi Yan. et al. Establishment of a chronic insomnia rat model of sleep fragmentation using unstable platforms surrounded by water. EXP THER MED. 2023 May;25(5):1-12} {IF} {Rat}
[IF={{ 2.5 }}] {Mohammad Saber Ebrahimi. et al. Orexin-A and BDNF in the Hippocampus of Middle-Aged Rats: Beneficial Effects of integrating Voluntary Physical Activity with Intermittent Fasting. PHYSIOL BEHAV. 2025 Jun;:115009} {WB} {Rat}
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Orexin A Rabbit pAb(bs-15509R)-50ul/100ul
￥1180 - 1980
