Tissue/cell: rat myocardium tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NPY1R Polyclonal Antibody, Unconjugated(bs-1070R) 1:400, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
- ￥1180 - 1980
- Bioss
- bs-1070R
- 2025年10月24日
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50ul/100ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 产品编号 | bs-1070R |
| 英文名称 | NPY1R Rabbit pAb |
| 中文名称 | 神经肽Y1受体抗体 |
| 英文别名 | neuropeptide Y1 receptor; Npy1r; neuropeptide Y receptor Y1; MGC109393; NPY-1; NPY1R_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human NPY1R |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 44 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Blood > Blood Pressure regulation Cardiovascular > Vasculature > Vasodilation Signal Transduction > Signaling Pathway > G Protein Signaling > GPCR |
| 亚细胞定位 | Cell membrane. |
| 相似性 | Belongs to the G-protein coupled receptor 1 family. |
| 功能 | Receptor for neuropeptide Y and peptide YY. The rank order of affinity of this receptor for pancreatic polypeptides is NPY > [Pro-34] PYY, PYY and [Leu-31, Pro-34] NPY > NPY (2-36) > [Ile-31, Gln-34] PP and PYY (3-36) > PP > NPY free acid. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Neuropeptide Y (NPY) is one of the most abundant neuropeptides in the mammalian nervous system and exhibits a diverse range of important physiologic activities, including effects on psychomotor activity, food intake, regulation of central endocrine secretion, and potent vasoactive effects on the cardiovascular system. There are two major subtypes of NPY (Y1 and Y2). The NPY Y1 receptors have been identified in a variety of tissues, including brain, spleen, small intestine, kidney, testis, placenta, breast, colon, ganglion, heart, and aortic smooth muscle. The Y2 receptor is found mainly in the central nervous system. NPY receptors are a member of the G protein-coupled receptor superfamily. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {ELISA} | {1:5000-10000} |
Sample:
Lane 1: Mouse NIH/3T3 tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Lane 3: Rat Kidney tissue lysates
Lane 4: Human 293T cell lysates
Primary: Anti-NPY1R (bs-1070R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 60 kDa
Lane 1: Mouse NIH/3T3 tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Lane 3: Rat Kidney tissue lysates
Lane 4: Human 293T cell lysates
Primary: Anti-NPY1R (bs-1070R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 60 kDa
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文献和实验该产品被引用文献
[IF={{ 6.2 }}] {Xuejun Yuan. et al.Multi-omics analysis explore the mechanism of deoxynivalenol inhibiting rabbit appetite through microbial-gut-brain axis..ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY.2025 Feb:291:117849.} {Western blot} {Rabbit}
[IF={{ 5.711 }}] {Kang X et al. Neuropeptide Y Acts Directly on Cartilage Homeostasis and Exacerbates Progression of Osteoarthritis through NPY2R. J Bone Miner Res. 2020 Feb 26.} {IHC,WB} {mouse}
[IF={{ 5.682 }}] {Tüfekci, Kıymet Kübra. et al. A Maternal High-Fat Diet Causes Anxiety-Related Behaviors by Altering Neuropeptide Y1 Receptor and Hippocampal Volumes in Rat Offspring: the Potential Effect of N-Acetylcysteine. MOL NEUROBIOL. 2022 Dec;:1-16} {IHC} {Rat}
[IF={{ 2.8 }}] {Jinsong Liu. et al.Identification and multi-dimensional validation of mitochondrial permeability transition-driven necrosis-related model to assess the prognosis and immunotherapy value in breast cancer.EUROPEAN JOURNAL OF MEDICAL RESEARCH.2025 Feb 18;30(1):113.} {Ihc} {Rabbit}
[IF={{ 13 }}] {Qihang Sun. et al.Mettl3-m6A-NPY axis governing neuron–microglia interaction regulates sleep amount of mice.CELL DISCOVERY.2025 Feb 4;11(1):10.} {IF} {Mouse}
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NPY1R Rabbit pAb(bs-1070R)-50ul/100ul
￥1180 - 1980
