Sample:
MDA-MB-231(Human) Cell Lysate at 40 ug
Primary: Anti-IL12 (bs-1789R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 34 kD
- ￥1180 - 2800
- Bioss
- bs-1789R
- 2025年10月24日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
phospho-VAV1 (Tyr174) Rabbit pAb(bs-5594R)-50ul/100ul/200ul
￥1180PTEN Mouse mAb(bsm-33320M)-50ul/100ul/200ul/200ug
￥1180
HOPX/LAGY Rabbit pAb, PE-Cy7 conjugated(bs-9462R-PE-Cy7)-100ul
￥2980
SARS-CoV-2 (2019-nCoV) Spike Neutralizing Mouse mAb(bsm-41627M)-100ug/200ug
￥3600SMAD7 Rabbit pAb, APC-Cy5.5 conjugated(bs-23328R-APC-Cy5.5)-100ul
￥2980
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1789R |
| 英文名称 | IL12A Rabbit pAb |
| 中文名称 | 白介素12抗体 |
| 英文别名 | IL12A_HUMAN; Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit(CLMF p35); IL-12 subunit p35; NK cell stimulatory factor chain 1(NKSF1); NKSF1; interleukin 12A; P35; CLMF; NFSK; NKSF1; |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human IL-12 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 23 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Tumor immunology > Cytokines > Interleukins Immunology > Innate Immunity > Cytokines > Interleukins |
| 亚基 | Heterodimer with IL12B; disulfide-linked. The heterodimer is known as interleukin IL-12. |
| 亚细胞定位 | Secreted. |
| 相似性 | Belongs to the IL-6 superfamily. |
| 功能 | Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | bs-0767P is one synthetic peptide derived from mouse IL-12. IL-12 protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: human lung carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-IL12 Polyclonal Antibody, Unconjugated(bs-1789R) 1:500, overnight at 4∑C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37∑C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-IL12 Polyclonal Antibody, Unconjugated(bs-1789R) 1:500, overnight at 4∑C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37∑C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
Tissue/cell: human colon carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-IL12 Polyclonal Antibody, Unconjugated(bs-1789R) 1:500, overnight at 4∑C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37∑C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-IL12 Polyclonal Antibody, Unconjugated(bs-1789R) 1:500, overnight at 4∑C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37∑C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
Sample: U251 Cell Lysate at 30 ug
Primary: Anti- IL12 (bs-1789R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 34 kD
Primary: Anti- IL12 (bs-1789R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 34 kD
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
[IF={{ 6.218 }}] {Foulon M et al. Mycolactone toxin induces an inflammatory response by targeting the IL-1β pathway: Mechanistic insight into Buruli ulcer pathophysiologyPLoS Pathog.2020 Dec 18;16(12):e1009107.} {IHC} {Mouse}
[IF={{ 3.8 }}] {Yuquan Guo. et al. Revealing the Mechanism: The Influence of Baicalin on M1/M2 and Th1/Th2 Imbalances in Mycoplasma gallisepticum Infection. POULTRY SCI. 2024 Jul;:104145} {WB} {Chicken}
[IF={{ 2.33 }}] {Li, Kang, et al. "CD14 overexpression upregulates TNF-??-mediated inflammatory responses and suppresses the malignancy of gastric carcinoma cells."Molecular and cellular biochemistry 376.1-2 (2013): 137-143.3} {WB} {="Human"}
相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
.g., Sigma) 50 mg/ml bis‐sulfosuccinimdylsuberate (BS3 ;Pierce, cat. no. PI 21580) in dimethylsulfoxide (DMSO) 5 mg/ml
技术资料暂无技术资料 索取技术资料
文献支持
IL12A Rabbit pAb(bs-1789R)-50ul/100ul/200ul
￥1180 - 2800
