Sample:
_x000D_
MDA-MB-231(Human) Cell Lysate at 40 ug
_x000D_
Primary: Anti-IL12 (bs-1789R) at 1/300 dilution
_x000D_
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_
Predicted band size: 23 kD
_x000D_
Observed band size: 34 kD
- ￥1180 - 2800
- Bioss
- bs-1789R
- 2026年10月13日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1789R |
| 英文名称 | IL12A Rabbit pAb |
| 中文名称 | 白介素12抗体 |
| 英文别名 | IL12A_HUMAN; Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit(CLMF p35); IL-12 subunit p35; NK cell stimulatory factor chain 1(NKSF1); NKSF1; interleukin 12A; P35; CLMF; NFSK; NKSF1; |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human IL-12 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 23 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Tumor immunology > Cytokines > Interleukins Immunology > Innate Immunity > Cytokines > Interleukins |
| 亚基 | Heterodimer with IL12B; disulfide-linked. The heterodimer is known as interleukin IL-12. |
| 亚细胞定位 | Secreted. |
| 相似性 | Belongs to the IL-6 superfamily. |
| 功能 | Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | bs-0767P is one synthetic peptide derived from mouse IL-12. IL-12 protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: human lung carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
_x000D_ Incubation: Anti-IL12 Polyclonal Antibody, Unconjugated(bs-1789R) 1:500, overnight at 4∑C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37∑C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
_x000D_ _x000D_
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
_x000D_ Incubation: Anti-IL12 Polyclonal Antibody, Unconjugated(bs-1789R) 1:500, overnight at 4∑C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37∑C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
_x000D_ _x000D_
Tissue/cell: human colon carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
_x000D_ Incubation: Anti-IL12 Polyclonal Antibody, Unconjugated(bs-1789R) 1:500, overnight at 4∑C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37∑C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
_x000D_
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
_x000D_ Incubation: Anti-IL12 Polyclonal Antibody, Unconjugated(bs-1789R) 1:500, overnight at 4∑C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37∑C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
_x000D_
Sample: U251 Cell Lysate at 30 ug
_x000D_ Primary: Anti- IL12 (bs-1789R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 23 kD
_x000D_ Observed band size: 34 kD
_x000D_
_x000D_ Primary: Anti- IL12 (bs-1789R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 23 kD
_x000D_ Observed band size: 34 kD
_x000D_
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IL12A | IL-12A Rabbit pAb(bs-1789R)-50ul/100ul/200ul
￥1180 - 2800
