COX4I1 | COX4 Rabbit pAb(bs-1533R)-50ul/100ul/200ul产品图
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COX4I1 | COX4 Rabbit pAb(bs-15

33R)-50ul/100ul/200ul
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  • ￥1180 - 2800
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  • bs-1533R
  • 2026年10月13日
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    北京博奥森生物技术有限公司
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    • 规格：

      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-1533R
    英文名称COX4I1 Rabbit pAb
    中文名称细胞色素c氧化酶IV亚型1抗体
    英文别名COX41_HUMAN; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; COX4; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1(COX IV-1); COX-IV; COX IV; COX 4; COX 4I1;
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse (Rat, Dog, Pig, Cow, Horse)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human COX4I1
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量17 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cancer > Cancer Metabolism > Metabolic signaling pathway > Integration of energy metabolism

    Isotype/Loading Controls > Loading Controls > COX IV - Mitochondrial

    Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Integration of energy

    Metabolism > Pathways and Processes > Mitochondrial Metabolism > Mitochondrial markers

    Metabolism > Pathways and Processes > Mitochondrial Metabolism > Oxidative phosphorylation > Complex IV

    Signal Transduction > Metabolism > Mitochondrial

    Tags & Cell Markers > Subcellular Markers > Organelles > Mitochondria

    亚细胞定位Mitochondrion inner membrane.
    组织特异性Ubiquitous.
    翻译后修饰Ubiquitous.
    相似性Belongs to the cytochrome c oxidase IV family.
    功能This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neiG‌‌HBor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq, Jul 2008]

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    Sample:
    _x000D_ heart (Mouse) Lysate at 40 ug
    _x000D_ Primary: Anti-COX4 (Bs-1533R) at 1/300 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 17 kD
    _x000D_ Observed band size: 17 kD
    产品细节图片2
    Tissue/cell: mouse brain tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    _x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    _x000D_ Incubation: Anti-COX4/COX IV-1 Polyclonal Antibody, Unconjugated(bs-1533R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    _x000D_
    产品细节图片3
    Sample:
    kidney (Mouse) Lysate at 40 ug
    Primary: Anti-COX4 (Bs-1533R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 17 kD
    Observed band size: 17 kD
    产品细节图片4
    Sample:
    _x000D_ Lane 1: Human Raji cell lysates
    _x000D_ Lane 2: Human HepG2 cell lysates
    _x000D_ Lane 3: Human MCF-7 cell lysates
    _x000D_ Lane 4: Human A431 cell lysates
    _x000D_ Primary: Anti-COX4I1 (bs-1533R) at 1/1000 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 17 kDa
    _x000D_ Observed band size: 15 kDa
    _x000D_ _x000D_

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    图标文献和实验
    该产品被引用文献
    [1]Cytoplasmic upregulation of Cyto c and AIF serve as biomarkers of mechanical asphyxia death.
    PMID:31396361Am J Transl Res2019-07-15
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    COX4I1 | COX4 Rabbit pAb(bs-1533R)-50ul/100ul/200ul
    ￥1180 - 2800