Tissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IGBP1 Polyclonal Antibody, Unconjugated(bs-0298R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-0298R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0298R |
| 英文名称 | IGBP1 Rabbit pAb |
| 中文名称 | 免疫球蛋白结合蛋白-1抗体 |
| 英文别名 | alpha 4; B cell signal transduction molecule alpha 4; B-cell signal transduction molecule alpha 4; bA351K23.1; CD79a binding protein 1; CD79a-binding protein 1; IBP 1; IBP1; IGBP 1; Igbp1; IGBP1_HUMAN; Immunoglobulin(CD79A) binding protein 1; Immunoglobulin binding protein 1; Immunoglobulin-binding protein 1; Protein alpha 4; Protein alpha-4; Protein phosphatase 2A regulatory subunit alpha 4; Renal carcinoma antigen NY REN 16; Renal carcinoma antigen NY-REN-16. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse, Pig, Cow, GuineaPig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Immunoglobulin binding protein 1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 44 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Protein Phosphorylation > Ser / Thr Phosphatases |
| 亚基 | Associates with PP2A-alpha and PP2A-beta catalytic subunits, and with PP4 and PP6. Interacts with MID1 and MID2. Interacts with Ubiquitin. |
| 亚细胞定位 | Cytoplasm (Potential). |
| 组织特异性 | Ubiquitously expressed with highest levels in heart, skeletal muscle and pancreas. |
| 翻译后修饰 | Phosphorylated.
Monoubiquitination by MID1 triggers calpain-mediated cleavage and switches IGBP1 activity from protective to destructive. |
| 相似性 | Belongs to the IGBP1/TAP42 family.
Contains 1 UIM (ubiquitin-interacting motif) repeat. |
| 功能 | Associated to surface IgM-receptor; may be involved in signal transduction. Involved in regulation of the catalytic activity of PP2A, PP4 and PP6 phosphatases catalytic subunits by protecting them from degradative polyubiquitination until they associate with regulatory subunits. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The proliferation and differentiation of B cells is dependent upon a B-cell antigen receptor (BCR) complex. Binding of antigens to specific B-cell receptors results in a tyrosine phosphorylation reaction through the BCR complex and leads to multiple signal transduction pathways. [provided by RefSeq, Jul 2008]. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1μg/Test} |
Blank control（black line）:HepG2.
Primary Antibody (green line): Rabbit Anti-IGBP1 antibody (bs-0298R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-IGBP1 antibody (bs-0298R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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文献和实验该产品被引用文献
[IF={{ 5.9 }}] {Duliurui Huang. et al. Analysis of the heterogeneity and complexity of murine extraorbital lacrimal gland via single-cell RNA sequencing. OCUL SURF. 2024 Jun;:} {IF} {Mouse}
[IF={{ 4.21 }}] {Luo, Guangying, et al. "Paternal bisphenol a diet changes prefrontal cortex proteome and provokes behavioral dysfunction in male offspring." Chemosphere (2017).} {WB} {="Mouse"}
[IF={{ 2.515 }}] {Li T et al. Digital gene expression analyses of mammary glands from meat ewes naturally infected with clinical mastitis. R. Soc. open sci. 2019,6: 181604.} {IHC} {Sheep}
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IGBP1 Rabbit pAb(bs-0298R)-50ul/100ul/200ul
￥1180 - 2800
