Tissue/cell: human stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CYP11B2 Polyclonal Antibody, Unconjugated(bs-10161R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-10161R
- 2025年10月16日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-10161R |
| 英文名称 | CYP11B2 Rabbit pAb |
| 中文名称 | 醛固酮合成酶CYP11B2抗体 |
| 英文别名 | CYP 11B2; CYPXI11B2; Cytochrome P450 1111B2; Cytochrome P450 1111B2 mitochondrial; Cytochrome P450 family 11 subfamily B polypeptide 2; Cytochrome P450 subfamily XIB(cholesterol side chain cleavage); Cytochrome P450 subfamily XI11B2; Cytochrome P450C1111B2; C11B2_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CYP11B2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 58 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Lipids / Lipoproteins > Lipid Metabolism > Cholesterol Metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Cholesterol Metabolism |
| 亚细胞定位 | Mitochondrion membrane. |
| 相似性 | Belongs to the cytochrome P450 family. |
| 功能 | Preferentially catalyzes the conversion of 11-deoxycorticosterone to aldosterone via corticosterone and 18-hydroxycorticosterone. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane. The enzyme has steroid 18-hydroxylase activity to synthesize aldosterone and 18-oxocortisol as well as steroid 11 beta-hydroxylase activity. Mutations in this gene cause corticosterone methyl oxidase deficiency. [provided by RefSeq, Jul 2008]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
Lane 1: Adrenal gland (Mouse) Tissue Lysate at 40 ug
Lane 2: Adrenal gland (Rat) TissueLysate at 40 ug
Primary:
Anti-CYP11B2 (bs-10161R) at 1/1000 dilution
Anti-beta-Actin (bs-0061R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 60 kD
Lane 1: Adrenal gland (Mouse) Tissue Lysate at 40 ug
Lane 2: Adrenal gland (Rat) TissueLysate at 40 ug
Primary:
Anti-CYP11B2 (bs-10161R) at 1/1000 dilution
Anti-beta-Actin (bs-0061R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 60 kD
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文献和实验该产品被引用文献
[IF={{ 4 }}] {Jing-Hang Jiang. et al.Human-like adrenal features in Chinese tree shrews revealed by multi-omics analysis of adrenal cell populations and steroid synthesis.zool res.2024 May 18;45(3):617-632.} {IHC} {Mouse}
[IF={{ 2.9 }}] {Mei You. et al.Meriones unguiculatus serves as a spontaneous primary aldosteronism rodent model.plos one.2025 Feb 13;20(2):e0314943.} {IHC} {Rat}
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CYP11B2 Rabbit pAb(bs-10161R)-50ul/100ul/200ul
￥1180 - 2800
