Tissue/cell: human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IRAK4 Polyclonal Antibody, Unconjugated(bs-2440R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-2440R
- 2026年05月21日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
Anti-human IL-33 (Tozorakimab Biosimilar)(BIO0799SM)-1mg/5mg/20mg
￥3200
H2AX (Ubiquityl Lys119) Rabbit pAb, APC conjugated(bs-8571R-APC)-100ul
￥2980
Elastin Rabbit pAb, FITC conjugated(bs-11057R-FITC)-100ul
￥2980calcium-activated chloride channel CLCA4 Rabbit pAb, AP conjugated(bs-1585R-AP)-100ul
￥2980ICAM5 Rabbit pAb, BF594 conjugated(bs-6686R-BF594)-100ul
￥2980
万千商家帮你免费找货
0 人在求购买到急需产品
相关阅读
源叶现货试剂/标准品，库存足，周末发货Cytoscape 教程来了！快速实现顶刊同款通路网络图五大应用案例：Mustang Q 膜层析应用全解析1 个小工具，一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-2440R |
| 英文名称 | IRAK4 Rabbit pAb |
| 中文名称 | 白介素-1受体相关激酶4抗体 |
| 英文别名 | IL-1 Receptor-associated Kinase 4; 8430405M07Rik; 9330209D03Rik; IPD1; IRAK4; NY-REN-64; REN64; INTERLEUKIN RECEPTOR-ASSOCIATED KINASE 4; Interleukin 1 receptor associated kinase 4 mutant form 1; Interleukin-1 receptor-associated kinase 4; Interleukin1 receptor associated kinase 4; IPD1; IRAK 4; IRAK-4; IRAK4 mutated form 1; IRAK4_HUMAN; LOC 51135; NY REN 64 antigen; Renal carcinoma antigen NY-REN-64. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Dog, Pig, Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human IRAK4 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 51 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Atherosclerosis > Vascular Inflammation > Inflammatory mediators Immunology > Innate Immunity > Cytokines > Interleukins Immunology > Innate Immunity > TLR Signaling Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > Other Kinases |
| 亚基 | Associates with MYD88 and IRAK2 to form a ternary complex called the Myddosome. Once phosphorylated, IRAK4 dissociates from the receptor complex and then associates with the TNF receptor-associated factor 6 (TRAF6), IRAK1, and PELI1; this intermediate complex is required for subsequent NF-kappa-B activation. Interacts with IL1RL1. |
| 亚细胞定位 | Cytoplasm. |
| 翻译后修饰 | Phosphorylated. |
| 相似性 | Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 death domain. Contains 1 protein kinase domain. |
| 功能 | Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IRAK4 Polyclonal Antibody, Unconjugated(bs-2440R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IRAK4 Polyclonal Antibody, Unconjugated(bs-2440R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Sample:
Lane 1: Muscle (Mouse) Lysate at 40 ug
Lane 2: Stomach (Mouse) Lysate at 40 ug
Lane 3: Muscle (Rat) Lysate at 40 ug
Primary: Anti-IRAK4 (bs-2440R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51-55 kD
Observed band size: 51 kD
Lane 1: Muscle (Mouse) Lysate at 40 ug
Lane 2: Stomach (Mouse) Lysate at 40 ug
Lane 3: Muscle (Rat) Lysate at 40 ug
Primary: Anti-IRAK4 (bs-2440R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51-55 kD
Observed band size: 51 kD
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
单抗（canakinumab）。这三种IL-1靶向药物在多种炎症性疾病中展现出良好疗效，安全性总体相似，主要不良反应为轻度非机会性感染风险增加，通常无需停药即可控制。 针对IL-1通路的小分子抑制剂正广泛研发。NLRP3炎症小体作为IL-1β活化关键节点，是主要靶点，如MCC950可有效抑制其激活，降低感染风险。上游靶向P2X7受体或下游靶向caspase-1、IRAK4、MK2等信号分子的抑制剂也在探索中，旨在阻断IL-1生成或信号传导。尽管部分药物因副作用受限，但小分子药物具穿透血脑屏障等优势，为治疗IL-1介导
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
技术资料暂无技术资料 索取技术资料
文献支持
IRAK4 Rabbit pAb(bs-2440R)-50ul/100ul/200ul
￥1180 - 2800
