Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_
Incubation: Anti-SSTR3 Polyclonal Antibody, Unconjugated(bs-1368R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
- ￥1180 - 2800
- Bioss
- bs-1368R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1368R |
| 英文名称 | SSTR3 Rabbit pAb |
| 中文名称 | 生长抑素受体3抗体 |
| 英文别名 | SSR3_HUMAN; Somatostatin receptor type 3; SS-3-R; SS3-R; SS3R; SST3; SSR-28; |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Dog, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human SSTR3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 46 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cell Death > Apoptosis > Receptors > Associated Proteins Cell Biology > Apoptosis > Receptors > Associated Proteins Cell Biology > Cell Cycle > Cell Cycle Inhibitors Signal Transduction > Second Messenger > Nucleotide Messenger > cGMP Signal Transduction > Signaling Pathway > G Protein Signaling > GPCR |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. Note=Internalized into endoplasmic vesicles upon somatostatin-stimulation. |
| 组织特异性 | Brain, pituitary and pancreas. |
| 翻译后修饰 | Phosphorylated. Phosphorylation increases upon somatostatin binding. |
| 相似性 | Belongs to the G-protein coupled receptor 1 family. |
| 功能 | Receptor for somatostatins-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the somatostatin receptor protein family. Somatostatins are peptide hormones that regulate diverse cellular functions such as neurotransmission, cell proliferation, and endocrine signaling as well as inhibiting the release of many hormones and other secretory proteins. Somatostatin has two active forms of 14 and 28 amino acids. The biological effects of somatostatins are mediated by a family of G-protein coupled somatostatin receptors that are expressed in a tissue-specific manner. Somatostatin receptors form homodimers and heterodimers with other members of the superfamily as well as with other G-protein coupled receptors and receptor tyrosine kinases. This protein is functionally coupled to adenylyl cyclase. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: Mouse pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-SSTR3 Polyclonal Antibody, Unconjugated(bs-1368R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-SSTR3 Polyclonal Antibody, Unconjugated(bs-1368R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
Sample:
_x000D_ Lane 1: Mouse Cerebrum tissue lysates
_x000D_ Lane 2: Mouse Cerebellum tissue lysates
_x000D_ Lane 3: Rat Cerebrum tissue lysates
_x000D_ Lane 4: Human U87MG cell lysates
_x000D_ Primary: Anti-SSTR3 (bs-1368R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 46 kDa
_x000D_ Observed band size: 46 kDa
_x000D_ Lane 1: Mouse Cerebrum tissue lysates
_x000D_ Lane 2: Mouse Cerebellum tissue lysates
_x000D_ Lane 3: Rat Cerebrum tissue lysates
_x000D_ Lane 4: Human U87MG cell lysates
_x000D_ Primary: Anti-SSTR3 (bs-1368R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 46 kDa
_x000D_ Observed band size: 46 kDa
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SSTR3 Rabbit pAb(bs-1368R)-50ul/100ul/200ul
￥1180 - 2800
