GFRA1 | GFR-alpha-1 Rabbit pAb(bs-0201R)-50ul/100ul/200ul产品图
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GFRA1 | GFR-alpha-1 Rabbit pAb

(bs-0201R)-50ul/100ul/200ul
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  • ¥1180 - 2800
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  • bs-0201R
  • 2026年10月13日
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      50ul/100ul/200ul

    规格:50ul产品价格:¥1180.0
    规格:100ul产品价格:¥1980.0
    规格:200ul产品价格:¥2800.0
    产品编号bs-0201R
    英文名称GDNFRA Rabbit pAb
    中文名称GDNF家族受体α-1抗体
    英文别名GDNF Receptor alpha 1; GFRa-1; GFR alpha; GDNFR alpha; TmR1; GFR alpha-1; RETL1; RET ligand 1; TGF-beta related neurotrophic factor receptor-1; AU042498; DKFZp313E1012; DKFZp686J0156; FLJ10561; FLJ31546; GDNFR; Gdnfr alpha; GFR-ALPHA-1; GFRA1; GRFA1; LOC143381; MGC23045; Ret; RET1L; RETL1; TRNR1; GFRA1_HUMAN; GDNF family receptor alpha-1; GDNF receptor alpha-1; GDNFR-alpha-1; TGF-beta-related neurotrophic factor receptor 1.
    中文别名TGF-β related neurotrophic factor receptor-1;
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat, Cerebrum, Human duodenum, Human duodenum (Dog, Pig, Cow, Horse)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human GFR Alpha-1
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量44 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Neuroscience > Neurology process > Growth and Development > Neurotrophins

    亚基2 molecules of GDNFR-alpha are thought to form a complex with the disulfide-linked GDNF dimer and with 2 molecules of RET.
    亚细胞定位Cell membrane; Lipid-anchor, GPI-anchor.
    相似性Belongs to the GDNFR family.
    功能Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq]

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    Tissue/cell: Human colon cancer ; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    _x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    _x000D_ Incubation: Anti-GDNFRA Polyclonal Antibody, Unconjugated(bs-0201R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    _x000D_
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GDNFRA) Polyclonal Antibody, Unconjugated (bs-0201R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded (Human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GDNFRA) Polyclonal Antibody, Unconjugated (bs-0201R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片4
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GDNFRA) Polyclonal Antibody, Unconjugated (bs-0201R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片5
    Sample:
    _x000D_ Lane 1: Mouse Cerebrum tissue lysates
    _x000D_ Lane 2: Mouse Testis tissue lysates
    _x000D_ Lane 3: Rat Cerebrum tissue lysates
    _x000D_ Primary: Anti-GDNFRA (bs-0201R) at 1/1000 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 44 kDa
    _x000D_ Observed band size: 47 kDa
    产品细节图片6
    Sample:
    _x000D_ Lane 1: Human HeLa cell lysates
    _x000D_ Lane 2: Human HepG2 cell lysates
    _x000D_ Lane 3: Human MOLT4 cell lysates
    _x000D_ Lane 4: Human A549 cell lysates
    _x000D_ Primary: Anti-GDNFRA (bs-0201R) at 1/1000 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 44 kDa
    _x000D_ Observed band size: 47 kDa

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    GFRA1 | GFR-alpha-1 Rabbit pAb(bs-0201R)-50ul/100ul/200ul
    ¥1180 - 2800