Sample: Breast milk (Human) Lysate at 40 ug
Primary: Anti-Lactoferrin (bs-5810R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 76 kD
Observed band size: 76 kD
- ￥1180 - 2800
- Bioss
- bs-5810R
- 2025年10月16日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-5810R |
| 英文名称 | Lactoferrin Rabbit pAb |
| 中文名称 | 乳铁蛋白抗体 |
| 英文别名 | CKRX; GIG12; Growth inhibiting protein 12; HLF2; Homo sapiens chemokine receptor X; Kaliocin 1; Lactoferrin; Lactoferroxin A; Lactoferroxin B; Lactoferroxin C; Lactoferroxin-C; Lactotransferrin; LF; LTF; Neutrophil lactoferrin; Talalactoferrin alfa; Talalactoferrin; TRFL_HUMAN. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=1ug/test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Dog, Pig, Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Lactoferrin |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 76 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cell Death > Apoptosis Cancer > Invasion/microenvironment > Apoptosis Cardiovascular > Blood > Serum Proteins |
| 亚基 | Monomer. Found in a complex with LTF, CLU, EPPIN and SEMG1. |
| 亚细胞定位 | Isoform 1: Secreted. Isoform DeltaLf: Cytoplasm. Nucleus. |
| 翻译后修饰 | Isoform DeltaLf: Ubiquitinated at Lys-379 and Lys-391. |
| 相似性 | Belongs to the transferrin family.
Contains 2 transferrin-like domains. |
| 功能 | Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration. Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors. The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity. Isoform DeltaLf: transcription factor with antiproliferative properties and inducing cell cycle arrest. Binds to DeltaLf response element found in the SKP1, BAX, DCPS, and SELH promoters.extracellular cation concentration. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration. Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors. The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {1ug/test} |
Blank control: U-937.
Primary Antibody (green line): Rabbit Anti-Lactoferrin antibody (bs-5810R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-Lactoferrin antibody (bs-5810R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Lactoferrin Antibody(bs-5810R) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed.Cells stained with primary antibody (green), and isotype control (orange).
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文献和实验该产品被引用文献
[IF={{ 8.109 }}] {Carman Man et al. Aging-Associated Alterations in Mammary Epithelia and Stroma Revealed by Single-Cell RNA SequencingCell Rep.2020 Dec 29;33(13):108566.} {IHC} {Mouse}
[IF={{ 15.992 }}] {Tsatsanis, Andrew. et al. The acute phase protein lactoferrin is a key feature of Alzheimer’s disease and predictor of Aβ burden through induction of APP amyloidogenic processing. Mol Psychiatr. 2021 Aug;:1-16} {IP} {Human}
[IF={{ 12.12 }}] {Zhao et al. Neutrophil polarization by IL-27 as a therapeutic target for intracerebral hemorrhage. (2017) Nat.Commun. 8:602} {WB} {Mouse, Rat}
[IF={{ 11.799 }}] {Zhongnan Xiao. et al. Reduction of lactoferrin aggravates neuronal ferroptosis after intracerebral hemorrhagic stroke in hyperglycemic mice. Redox Biol. 2022 Apr;50:102256} {IF,WB} {Mouse}
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Lactoferrin Rabbit pAb(bs-5810R)-50ul/100ul/200ul
￥1180 - 2800
