Sample:
_x000D_
Raw264.7(Mouse) Cell Lysate at 30 ug
_x000D_
Primary: Anti- CD16 (bs-6028R) at 1/1000 dilution
_x000D_
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_
Predicted band size: 27 kD
_x000D_
Observed band size: 50 kD
_x000D_
- ￥1180 - 2800
- Bioss
- bs-6028R
- 2026年10月13日
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- 文献和实验
- 技术资料
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-6028R |
| 英文名称 | CD16 Rabbit pAb |
| 中文名称 | FC段γ受体3/免疫球蛋白G Fc段受体III抗体 |
| 英文别名 | FCG3A_HUMAN; Low affinity immunoglobulin gamma Fc region receptor III-A; CD16a antigen; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc receptor III-2; CD_antigen: D16a; FCGR3A; CD16A; FCG3; FCGR3; IGFR3. |
| 产品应用 | WB=1:500-1000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human IGFR3/CD16 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 27 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Immunology > Cell Type Markers > CD > NK Cells Stem Cells > Hematopoietic Progenitors > Hematopoietic Stem Cells > Human Lineage Negative Stem Cells > Hematopoietic Progenitors > Lymphoid > NK Cell Lineage Stem Cells > Hematopoietic Progenitors > Myeloid > Monocytic Lineage Stem Cells > Hematopoietic Progenitors > Myeloid > Neutrophil Lineage |
| 亚基 | Exists as a heterooligomeric receptor complex with Fc epsilon receptor I gamma subunit and / or the CD3 zeta subunit. Interacts with INPP5D/SHIP1. |
| 亚细胞定位 | Cell membrane. Secreted. Exists also as a soluble receptor. |
| 组织特异性 | Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts. |
| 翻译后修饰 | Glycosylated. Contains high mannose- and complex-type oligosaccharides. The soluble form is produced by a proteolytic cleavage. [MISCELLANEOUS] Encoded by one of two nearly indentical genes: FCGR3A (Shown here) and FCGR3B which are expressed in a tissue-specific manner. The Phe-203 in III-A determines the transmembrane domains whereas the 'Ser-203' in III-B determines the GPI-anchoring. |
| 相似性 | Contains 2 Ig-like C2-type (immunoglobulin-like) domains. |
| 功能 | Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other responses, including antibody dependent cellular mediated cytotoxicity and antibody dependent enhancement of virus infections. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene are associated with immunodeficiency 20, and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2020] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-1000} |
Sample:
_x000D_ Lane 1: Thymus (Rat) Lysate at 40 ug
_x000D_ Lane 2: Lymph node (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-CD16 (bs-6028R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 55 kD
_x000D_ Observed band size: 60 kD
_x000D_ Lane 1: Thymus (Rat) Lysate at 40 ug
_x000D_ Lane 2: Lymph node (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-CD16 (bs-6028R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 55 kD
_x000D_ Observed band size: 60 kD
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FCGR3A | CD16a/FcγRIIIa Rabbit pAb(bs-6028R)-50ul/100ul/200ul
￥1180 - 2800
