Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NeuroD1 Polyclonal Antibody, Unconjugated(bs-1517R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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- 2025年10月16日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1517R |
| 英文名称 | NEUROD1 Rabbit pAb |
| 中文名称 | 神经细胞分化因子1抗体 |
| 英文别名 | atonal; Neurod1 protein; basic helix loop helix transcription factor; bHLHa3; class A basic helix loop helix protein 3; Class A basic helix-loop-helix protein 3; MODY 6; MODY6; NDF1_HUMAN; NeuroD1; neurogenic helix loop helix protein NEUROD; beta cell E box transactivator 2; BETA2; BHF 1; BHF1; NEUROD; Neurogenic differentiation 1; Neurogenic differentiation factor 1; NIDDM; BHLHA3. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2ug/test Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Dog, Pig, Cow) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human NEUROD1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 40 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Atherosclerosis > Diabetes associated Epigenetics and Nuclear Signaling > Transcription > Domain Families > HLH / Leucine Zipper > HLH Metabolism > Types of disease > Diabetes Metabolism > Types of disease > Heart disease Neuroscience > Cell Type Marker > Neural Stem Cell marker Neuroscience > Neurology process > Metabolism Neuroscience > Neurology process > Neurogenesis |
| 亚基 | Interacts (via helix-loop-helix motif domain) with EP300(via C-terminus) (By similarity). Heterodimer with TCF3/E47; theheterodimer is inhibited in presence of ID2, but not NR0B2, toE-box element. Efficient DNA-binding requires dimerization withanother bHLH protein. Interacts with RREB1. Interacts with EP300;the interaction is inhibited by NR0B2. Interacts with TCF3; theinteraction is inhibited by ID2. |
| 亚细胞定位 | Cytoplasm. Nucleus. |
| 翻译后修饰 | Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth. |
| 相似性 | Contains 1 basic helix-loop-helix (bHLH) domain. |
| 功能 | Differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and mutations in this gene result in type II diabetes mellitus. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {0.2ug/test} |
Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-Neuro D1/AF647 Conjugated antibody (bs-1517R-AF647)
Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-AF647.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-Neuro D1/AF647 Conjugated antibody (bs-1517R-AF647)
Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-AF647.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
Sample:
Lane 1: Stomach (Mouse) Lysate at 40 ug
Lane 2: Spinal cord (Mouse) Lysate at 40 ug
Primary: Anti-NeuroD1 (bs-1517R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Lane 1: Stomach (Mouse) Lysate at 40 ug
Lane 2: Spinal cord (Mouse) Lysate at 40 ug
Primary: Anti-NeuroD1 (bs-1517R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Sample:
Lane 1: Cerebellum (Mouse) Lysate at 40 ug
Lane 2: Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- NeuroD1 (bs-1517R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Lane 1: Cerebellum (Mouse) Lysate at 40 ug
Lane 2: Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- NeuroD1 (bs-1517R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
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NEUROD1 Rabbit pAb(bs-1517R)-50ul/100ul/200ul
￥1180 - 2800
