Sample:
_x000D_
Lane 1: Normal human Jurkat cell lysates
_x000D_
Lane 2: Jurkat treated with PMA 125ng/ml 30min
_x000D_
Primary: Anti-Integrin alpha 4 (bs-0641R) at 1/1000 dilution
_x000D_
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_
Predicted band size: 111 kDa
_x000D_
Observed band size: 150 kDa
_x000D_
- ￥1180 - 1980
- Bioss
- bs-0641R
- 2026年10月13日
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50ul/100ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 产品编号 | bs-0641R |
| 英文名称 | Integrin alpha 4 Rabbit pAb |
| 中文名称 | 整合素α4（CD49d）抗体 |
| 英文别名 | alpha 4 subunit of VLA 4 receptor; ITG-α4: Antigen CD49D; CD 49d; CD49d; CD49d antigen; CDw49d; IA4; Integrin alpha IV; ITGA 4; ITGA4; LPAM23; MGC90518; Very Late Activation Antigen 4; Very Late Activation Protein 4 Receptor alpha 4 Subunit; VLA 4; VLA4; integrin alpha-4; ITA4_HUMAN; Integrin α4; Integrin α 4. |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Integrin alpha 4 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 111 kDa |
| 实际分子量 | 150 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Angiogenesis > Endothelial Cell Markers Immunology > Cell Type Markers > CD > Non-lineage Signal Transduction > Cytoskeleton / ECM > Cell Adhesion > Integrins > Alpha Stem Cells > Neural Stem Cells > Surface Molecules |
| 亚基 | Heterodimer of an alpha and a beta subunit. |
| 亚细胞定位 | Membrane; Single pass type I membrane protein. |
| 翻译后修饰 | Phosphorylation on Ser-1027 inhibits PXN binding. |
| 相似性 | Belongs to the integrin alpha chain family._x000D_
Contains 7 FG-GAP repeats. |
| 功能 | Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Integrin alpha 4 beta 7, also known as, the lymphocyte Peyer's patch adhesion molecule (LPAM1) is a member of the integrin family of cell surface receptors. It is expressed primarily on mucosal lymphocytes, but is also present on NK cells and eosinophils. The alpha 4 beta 7 heterodimer mediates the binding of lymphocytes to its ligand, mucosal vascular addressin (MAdCAM1) on the high endothelial venules, thereby directing the homing of lymphocytes into Peyer's patches and intestinal lamina propria. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {ELISA} | {1:5000-10000} |
Blank control: U937(blue).
Primary Antibody: Rabbit Anti-Integrin alpha 4 antibody(bs-0641R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
_x000D_ _x000D_ Protocol
_x000D_ The cells were fixed with 2% Paraformaldehyde (10 min). Primary antibody (bs-0641R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed._x000D_
Primary Antibody: Rabbit Anti-Integrin alpha 4 antibody(bs-0641R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
_x000D_ _x000D_ Protocol
_x000D_ The cells were fixed with 2% Paraformaldehyde (10 min). Primary antibody (bs-0641R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed._x000D_
Black line : Positive blank control (U937); Negative blank control (MCF7）
_x000D_ Green line : Primary Antibody (Rabbit Anti-Integrin alpha 4 antibody (bs-0641R) )
_x000D_ Orange line：Isotype Control Antibody (Rabbit IgG) .
_x000D_ Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF647)
_x000D_ U937（Positive）and MCF7（Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Integrin alpha 4 Antibody(bs-0641R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange)._x000D_
_x000D_ Green line : Primary Antibody (Rabbit Anti-Integrin alpha 4 antibody (bs-0641R) )
_x000D_ Orange line：Isotype Control Antibody (Rabbit IgG) .
_x000D_ Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF647)
_x000D_ U937（Positive）and MCF7（Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Integrin alpha 4 Antibody(bs-0641R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange)._x000D_
Sample:
_x000D_ Lane 1: Human A549 cell lysates
_x000D_ Lane 2: Human MCF-7 cell lysates
_x000D_ Primary: Anti-Integrin alpha 4 (bs-0641R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 111 kDa
_x000D_ Observed band size: 150 kDa
_x000D_ _x000D_
_x000D_ Lane 1: Human A549 cell lysates
_x000D_ Lane 2: Human MCF-7 cell lysates
_x000D_ Primary: Anti-Integrin alpha 4 (bs-0641R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 111 kDa
_x000D_ Observed band size: 150 kDa
_x000D_ _x000D_
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ITGA4 | CD49d Rabbit pAb(bs-0641R)-50ul/100ul
￥1180 - 1980
