Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti- IGFBP5 (bs-0406R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD
- ￥1180 - 2800
- Bioss
- bs-0406R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0406R |
| 英文名称 | IGFBP5 Rabbit pAb |
| 中文名称 | 胰岛素样生长因子结合蛋白5抗体 |
| 英文别名 | IBP 5; IBP5; IGF binding protein 5; IGF BP5; Insulin Like Growth Factor Binding Protein 5; IBP5_HUMAN; Insulin-like growth factor-binding protein 5; IBP-5; IGF-binding protein 5; IGFBP-5. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Chicken, Dog, Pig, Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human IGFBP5 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 30 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Growth Factors/Hormones > Insulin / Insulin-like Signal Transduction > Signaling Pathway > Lipid Signaling |
| 亚细胞定位 | Secreted. |
| 组织特异性 | Osteosarcoma, and at lower levels in liver, kidney and brain. |
| 相似性 | Contains 1 IGFBP N-terminal domain. Contains 1 thyroglobulin type-1 domain. |
| 功能 | IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | IGFBP5 is one of a family of proteins that bind to insulin-like growth factors (IGFs). IGFBP5 is a multifunctional protein which acts not only as a traditional binding protein but also functions as a growth factor independent of IGFs to stimulate bone formation. Currently there are seven named IGF-BPs that form high affinity complexes with both IGFI and IGFII. IGFBP5 is the major IGF-binding protein present in bone tissue and helps potentiate the action of IGFI on smooth muscle cells, fibroblasts or osteoblasts. Data shows that IGFBP5 acts as a growth inhibitor and pro-apoptotic agent in breast cancer cells. IGFBP5 overexpressing mice show an increase in neonatal mortality, reduced female fertility, whole-body growth inhibition and retarded muscle development.Function : IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
Tissue/cell: human nasopharyngeal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IGFBP5/IBP5 Polyclonal Antibody, Unconjugated(bs-0406R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IGFBP5/IBP5 Polyclonal Antibody, Unconjugated(bs-0406R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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文献和实验该产品被引用文献
[IF={{ 3.3 }}] {Wu Cuiling. et al. Regulation of secondary hair follicle cycle in cashmere goats by miR-877-3p targeting IGFBP5 gene. J ANIM SCI. 2023 Sep;:} {WB} {Goat}
[IF={{ 2.8 }}] {Zhang Junrui. et al. Targeting miR-103a-3p/IGFBP5 axis: a potential therapeutic strategy for gastric cancer progression. Discover Oncology. 2025 Dec;16(1):1-12} {} {}
[IF={{ 0 }}] {Yamada, Koichi, et al. "Genetic and Phenotypic Changes of Thymus Tissue in Mice during Pregnancy." Journal of Oral Health and Biosciences 30.1 (2017): 26-32.} {IHC} {="Mouse"}
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IGFBP5 Rabbit pAb(bs-0406R)-50ul/100ul/200ul
￥1180 - 2800
