IFNG | IFN-gamma Mouse mAb(bsm-0388M)-50ul/100ul/200ul/200ug产品图
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IFNG | IFN-gamma Mouse mAb(bsm

-0388M)-50ul/100ul/200ul/200ug
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  • 2026年10月13日
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    北京博奥森生物技术有限公司
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    • 规格：

      50ul/100ul/200ul/200ug

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    规格：200ug产品价格：￥5600.0
    产品编号bsm-0388M
    英文名称IFN gamma(F2E4) Mouse mAb
    中文名称γ-干扰素/γ-IFN单克隆抗体
    英文别名IFNG; IFG; IFI; IFN gamma; IFN Immune; IFN-gamma; IFNG; IFNG_HUMAN; Immune Interferon; Interferon gamma; Interferon gamma Precursor; Macrophage Activating Factor; MAF; T Cell Interferon; Type II Interferon; hIFN-gamma.
    产品应用Flow-Cyt=1ug/Test, ICC/IF=1:50-200

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human
    抗体来源Mouse
    免疫原Recombinant Full length of human IFN gamma
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein G
    克隆类型Monoclonal
    理论分子量17 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cancer > Tumor immunology > Cytokines > Interferons

    Immunology > Adaptive Immunity > Regulatory T Cells

    Immunology > Innate Immunity > Cytokines > Interferons

    Stem Cells > Signaling Pathways > TGF beta > Interferon gamma

    亚基Homodimer.
    亚细胞定位Secreted.
    组织特异性Released primarily from activated T lymphocytes.
    翻译后修饰Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
    相似性Belongs to the type II (or gamma) interferon family.
    功能Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Mammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.

     

    应用推荐稀释比例
    {Flow-Cyt}{1ug/Test}
    {ICC/IF}{1:50-200}

     

    产品细节图片1
    The Jurkat（Treated with PMA (25 ng/mL, 6 h) and ionomycin (1 µg/mL, 6 h), BFA (5 µg/ml, last 5 h)） (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-IFN gamma antibody (bsm-0388M): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 μg/test.Blank control (black): PBS. Acquisition of 20,000 events was performed.
    产品细节图片2
    4% P‌‌araformaldehyde-fixed Jurkat（Treated with PMA (25 ng/mL, 6 h) and ionomycin (1 µg/mL, 6 h), BFA (5 µg/ml, last 5 h)）(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (IFN gamma) monoclonal Antibody, unconjugated (bsm-0388M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

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    图标文献和实验
    该产品被引用文献

    [IF=3.17] Zhang, Jia‐xiang, et al. "Complement C5a–C5aR interaction enhances MAPK signaling pathway activities to mediate renal injury in trichloroethylene sensitized BALB/c mice." Journal of Applied Toxicology (2015). IHC ="Mouse"

    相关实验

    • Cytokine Bioassays

      virus solution at a density of 1-4 x 104 pfu/ml (see Quick Guide Chart for human IFN-gamma: 1/250 dilution of stock of 107 pfu/ml, for mouse IFN-gamma, 1/1000 dilution of stock of 107 pfu/ml). Incubate plate for 40 hours at 37°C, 5% CO2

    • Foxp3: Treg的主要调节因子

      ，表现出Th1、Th2和TFH等具有传统功能的细胞亚群。导致Foxp3下调的关键因素包括高水平的炎症因子内环境，如可诱导效应性T细胞产生的细胞因子：IL-6和IFN-gamma。Tregs 细胞中Foxp3的表达分析CD4+CD25+ Treg 细胞中Foxp3 +细胞群表明与Foxp3+Treg完全重合。使用Foxp3鉴定Treg用Anti-Mouse CD4 FITC、Anti-Mouse CD25 APC和Anti-Mouse/Rat Foxp3 PE 标记BALB/c小鼠 脾细胞. 右上

    • Intracellular Cytokine Staining Protocol

      by flow cytometry since cytokine levels are typically too low in freshly-prepared cells. Stimulation of cells with the appropriate reagent will depend on the cell type and the experimental conditions. For example, to stimulate T cells to produce IFN-gamma

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    IFNG | IFN-gamma Mouse mAb(bsm-0388M)-50ul/100ul/200ul/200ug
    ￥1180 - 5600