Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GnRHR Polyclonal Antibody, Unconjugated(bs-1464R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- 北京
- bs-1464R
- 2025年10月15日
- Elisa=1:500-1000,IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500,ICC=1:100-500,
- Rabbit
- Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep,
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
KLH conjugated synthetic peptide derived from human GnRHR
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 保存条件：
Store at -20 °C for one year.
- 克隆性：
单克隆
- 标记物：
无
- 适应物种：
Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep,
- 保质期：
1年
- 目录编号：
bs-1464R
- 库存：
999
- 供应商：
北京博奥森生物技术有限公司
- 宿主：
Rabbit
- 应用范围：
Elisa=1:500-1000,IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500,ICC=1:100-500,
- 浓度：
1mg/ml
- 抗体英文名：
GnRHR
- 抗体名：
促性腺激素释放激素受体抗体
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.00 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.00 |
| 规格： | 200ul | 产品价格： | ￥2800.00 |
| 产品编号 | bs-1464R |
| 英文名称 | GnRHR Rabbit pAb |
| 中文名称 | 促性腺激素释放激素受体抗体 |
| 英文别名 | Gonadotropin-releasing hormone receptor; GH1; Lhrhr; GnRH receptor; gnrh-r; GnRHR; GNRHR1; Gonadotropin releasing hormone receptor; GRHR; leutinizing-releasing hormone receptor; lh-rh; LHRHR; LRHR; luteinizing hormone releasing hormone receptor; GNRHR_HUMAN |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Dog, Pig, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GnRHR |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 36 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Endocrine system > Gonadotrophic axis |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. |
| 组织特异性 | Pituitary, ovary, testis, breast and prostate but not in liver and spleen. |
| 相似性 | Belongs to the G-protein coupled receptor 1 family. |
| 功能 | Receptor for gonadotropin releasing hormone (GnRH) that mediates the action of GnRH to stimulate the secretion of the gonadotropic hormones luteinizing hormone (LH) and follicle-stimulating hormone (FSH). This receptor mediates its action by association with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Isoform 2 may act as an inhibitor of GnRH-R signaling. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Gonadotropin Releasing Hormone (GnRH) is down-regulated by hCG and believed to be an autocrine factor that regulates the ovary. The Gonadotropin Releasing Hormone Receptor (GnRHR) is synthesized in the pituitary gland. Activin A has been shown to stimulate the synthesis of GnRHR, illustrating a possible mechanism for the modulation of gonadotropin responsiveness to GnRH. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GnRHR Polyclonal Antibody, Unconjugated(bs-1464R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GnRHR Polyclonal Antibody, Unconjugated(bs-1464R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-GnRHR (bs-1464R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62-70 kD
Observed band size: 63 kD
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-GnRHR (bs-1464R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62-70 kD
Observed band size: 63 kD
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文献和实验该产品被引用文献
[IF={{ 1.39 }}] {Xiao, Liu, et al. "Distribution patterns and developmental changes of GnRH and GnRHR-immunopositive cells in the pituitary of Ji Ning gray goats." Pakistan Veterinary Journal 34.1 (2014): 116-119.} {IHC} {="Goat"}
[IF={{ 3.14 }}] {Liu, Te, et al. "Effects of di-(2-ethylhexyl) phthalate on the hypothalamus-pituitary-ovarian axis in adult female rats." Reproductive Toxicology (2014).} {IHC} {="Rat"}
[IF={{ 3.34 }}] {López-Doval, S., R. Salgado, and A. Lafuente. "The expression of several reproductive hormone receptors can be modified by perfluorooctane sulfonate (PFOS) in adult male rats." Chemosphere 155 (2016): 488-497.} {Other} {="Rat"}
[IF={{ 4.736 }}] {Lu Xiaosheng. et al. Deficiency of C1QL1 reduced murine ovarian follicle reserve through intraovarian and endocrine control. ENDOCRINOLOGY. 2022 Apr;:} {IHC} {Mouse}
[IF={{ 2.7 }}] {Zixuan Chen. et al. Therapeutic Effects of Melatonin in Female Mice with Central Precocious Puberty by Regulating the Hypothalamic Kiss-1/Kiss1R System. BEHAV BRAIN RES. 2023 Nov;:114783} {WB} {Mouse}
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anti-GnRHR
￥1180 - 2800
