Blank control (Black line): Molt4 (Black).
Primary Antibody (green line):Rabbit Anti-PGC1 alpha+beta antibody (bs-7535R)
Dilution:1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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- bs-7535R
- 2025年10月16日
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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-7535R |
| 英文名称 | PGC1 alpha + beta Rabbit pAb |
| 中文名称 | 过氧化物酶体增殖物激活受体γ辅激活子1α+β抗体 |
| 英文别名 | LEM6; Ligand effect modulator 6; PERC; Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha; Peroxisome proliferative activated receptor, gamma, coactivator 1; Peroxisome proliferator activated receptor gamma coactivator 1 alpha; peroxisome proliferator-activated receptor gamma coactivator 1 beta; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; Peroxisome proliferator-activated receptor gamma coactivator 1-beta; peroxisome proliferator-activated receptor gamma, coactivator 1 beta; PGC 1(alpha); PGC 1 alpha; PGC 1v; PGC-1(beta); PGC-1-alpha; PGC-1-beta; PGC-1-related estrogen receptor alpha coactivator; PGC1; PGC1(alpha); PGC1A; PGC1v; PPAR gamma coactivator 1 alpha; PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6; PPAR gamma coactivator 1; PPAR gamma coactivator variant form; PPAR gamma coactivator-1beta; PPAR-gamma coactivator 1-alpha; PPAR-gamma coactivator 1-beta; PPARGC 1 alpha; PPARGC-1-alpha; PPARGC-1-beta; PPARGC1; PPARGC1A; Ppargc1b; PRGC1_HUMAN; PRGC2_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse, Dog, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human PGC1 alpha + beta |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 88/113 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Atherosclerosis > Diabetes associated Cardiovascular > Lipids / Lipoproteins > Fatty Acids > Metabolism Epigenetics and Nuclear Signaling > Nuclear Signaling Pathways > Nuclear Receptors > Co-activators/co-repressors Epigenetics and Nuclear Signaling > Nuclear Signaling Pathways > Nuclear Receptors > Estrogen Epigenetics and Nuclear Signaling > Transcription > Other factors Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Energy Metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Fatty acids Metabolism > Pathways and Processes > Mitochondrial Metabolism > Mitochondrial markers Metabolism > Pathways and Processes > Redox metabolism > Fatty acid oxidation Metabolism > Types of disease > Diabetes Metabolism > Types of disease > Heart disease Metabolism > Types of disease > Obesity Signal Transduction > Metabolism > Energy Metabolism Signal Transduction > Metabolism > Mitochondrial |
| 亚基 | Binds MYBBP1A, which inhibits transcriptional activation by this protein. Interacts with PRDM16. Interacts with LRPPRC. Homooligomer. Interacts with LPIN1. |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects. |
| 翻译后修饰 | hosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2.
Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions. |
| 相似性 | Contains 1 RRM (RNA recognition motif) domain. |
| 功能 | Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity (referenced from entrez gene. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1μg/Test} |
Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PGC1 alpha+beta Polyclonal Antibody, Unconjugated(bs-7535R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PGC1 alpha+beta Polyclonal Antibody, Unconjugated(bs-7535R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Sample:
Lane 1: Molt-4 (Human) Cell Lysate at 30 ug
Lane 2: A431 (Human) Cell Lysate at 30 ug
Lane 3: HepG2 (Human) Cell Lysate at 30 ug
Lane 4: Hela (Human) Cell Lysate at 30 ug
Lane 5: Jurkat (Human) Cell Lysate at 30 ug
Lane 6: HL60 (Human) Cell Lysate at 30 ug
Primary: Anti-PGC1 alpha + beta (bs-7535R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 90/113 kD
Observed band size: 113 kD
Lane 1: Molt-4 (Human) Cell Lysate at 30 ug
Lane 2: A431 (Human) Cell Lysate at 30 ug
Lane 3: HepG2 (Human) Cell Lysate at 30 ug
Lane 4: Hela (Human) Cell Lysate at 30 ug
Lane 5: Jurkat (Human) Cell Lysate at 30 ug
Lane 6: HL60 (Human) Cell Lysate at 30 ug
Primary: Anti-PGC1 alpha + beta (bs-7535R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 90/113 kD
Observed band size: 113 kD
Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PGC1 alpha + beta) Polyclonal Antibody, Unconjugated (bs-7535R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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PGC1 alpha + beta Rabbit pAb(bs-7535R)-50ul/100ul/200ul
￥1180 - 2800
