Tissue/cell: rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
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Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
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Incubation: Anti-Somatostatin/GRIH Polyclonal Antibody, Unconjugated(bs-1132R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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- ￥1180 - 2800
- Bioss
- bs-1132R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1132R |
| 英文名称 | Somatostatin 14/Somatostatin 28 Rabbit pAb |
| 中文名称 | 生长抑素抗体 |
| 英文别名 | GRIH; Growth hormone release inhibiting factor; SMS_HUMAN; SMST; SST; Somatostatin 14; Somatostatin14; Somatostatin-14; somatostatin SS14; Somatostatin 28; Somatostatin28; Somatostatin-28; somatostatin SS28. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:200-800 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Chicken, Pig, Cow, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Somatostatin 28 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 13 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Developmental Biology > Lineage specification > Endoderm Neuroscience > Neurology process > Neuroendocrinology > GH Regulation Neuroscience > Neurotransmitter > Neuropeptides > Hormones Stem Cells > Lineage Markers > Endoderm |
| 亚细胞定位 | Secreted. |
| 相似性 | Belongs to the somatostatin family. |
| 功能 | The cyclic tetradecapeptide somatostatin is widely distributed throughout the body and is an important regulator of endocrine and nervous system function. It exerts its biologic actions by binding to specific high affinity receptors on the cell surface. Somatostatin inhibits the release of somatotropin. Somatostatin has also been shown in sympathetic nerves, mucosal cells, myenteric nerves of the gastrointestinal tract, salivary glands and in some parafollicular cells of the thyroid. Somatostatin positive cells may also be present in medullary thyroid carcinomas, C cell hyperplasia, thymic and lung tumors and pulmonary small cell carcinomas. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Somatostatin is a regulatory hormone that is expressed throughout the body and inhibits the release of numerous secondary hormones by binding to high-affinity G protein-coupled somatostatin receptors. This cyclic tetradecapeptide inhibits the secretion of many important hormones, including somatotropin (also designated growth hormone, or GH), insulin and glucagon. Somatostatin is found in both the hypothalamus and pancreas. Somatostatin is thought to be involved in the regulation of insulin synthesis. The hormone somatostatin has active 14 amino acid and 28 amino acid forms that are produced by alternate cleavage of the single preproprotein encoded by this gene. In the cerebellum, Somatostatin-14 and Somatostatin-28 are highly expressed at birth and in the adult stage, respectively. Somatostatin affects rates of neurotransmission in the central nervous system and proliferation of both normal and tumorigenic cells. The gene encoding Somatostatin maps to human chromosome 3q28. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:200-800} |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Somatostatin 28) Polyclonal Antibody, Unconjugated (bs-1132R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
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文献和实验该产品被引用文献
[IF=4.26] Okita et al. Modified Western blotting for insulin and other diabetes-associated peptide hormones. (2017) Sci.Re. 7:6949 WB Mouse
[IF=0.392] Yildiz, M.. et al. The Effect of Chronic Intermittent Cold Exposure on Gastrin-, Somatostatin-, Secretin-, and Serotonin-Containing Cells in the Small Intestine of Rats. Biol Bull+. 2021 Dec;48(3):S95-S106 IHC Rat
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SST | SST-14 Rabbit pAb(bs-1132R)-50ul/100ul/200ul
￥1180 - 2800
