Sample: Testis (Rat) Lysate at 30 ug
Primary: Anti-phospho-GSK-3 Beta(Ser9) (bs-2066R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size : 47kD
Observed band size : 49kD
- ￥1180 - 2800
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- bs-2066R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-2066R |
| 英文名称 | phospho-GSK-3 Beta (Ser9) Rabbit pAb |
| 中文名称 | 磷酸化糖原合酶激酶-3β抗体 |
| 英文别名 | GSK3 beta(phospho S9); p-GSK3 beta(phospho S9); GSK3B(phospho-Ser9); GSK3B(phospho-S9); p-GSK-3 beta(Ser9); p-GSK-3 beta(S9); Glycogen synthase kinase 3 beta; GSK 3 beta; GSK 3B; GSK3B; GSK3B protein; GSK3beta isoform; GSK3 beta; Glycogen synthase kinase-3 beta; GSK-3 beta; GSK3B_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, colon, colon, colon, colon, colon, colon, colon, Cerebrum, Cerebrum, Cerebrum |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Ser9 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 47 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Integration of energy metabolism Cancer > Cancer Metabolism > Metabolic signaling pathway > Metabolism of carbohydrates Cardiovascular > Atherosclerosis > Diabetes associated Cardiovascular > Heart > Hypertrophy Metabolism > Pathways and Processes > Metabolic signaling pathways > Carbohydrate metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Integration of energy Metabolism > Types of disease > Cancer Metabolism > Types of disease > Diabetes Metabolism > Types of disease > Heart disease Neuroscience > Neurology process > Notch Pathway Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > Other Kinases Stem Cells > Signaling Pathways > Notch > Cytoplasmic Stem Cells > Signaling Pathways > Wnt > Cytoplasmic |
| 亚基 | Monomer (By similarity). Interacts with ARRB2 and DISC1 (By similarity). Interacts with CABYR, MMP2, MUC1, NIN and PRUNE Interacts with AXIN1; the interaction mediates hyperphosphorylation of CTNNB1 leading to its ubiquitination and destruction. Interacts with and phosphorylates SNAI1. Interacts with DNM1L (via a C-terminal domain). Found in a complex composed of MACF1, APC, AXIN1, CTNNB1 and GSK3B (By similarity). Interacts with SGK3. |
| 亚细胞定位 | Cytoplasm. Nucleus. Cell membrane. Note=The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane. |
| 组织特异性 | Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. |
| 翻译后修饰 | Phosphorylated by AKT1 and ILK1. Upon insulin-mediated signaling, the activated PKB/AKT1 protein kinase phosphorylates and desactivates GSK3B, resulting in the dephosphorylation and activation of GYS1. Activated by phosphorylation at Tyr-216. |
| 相似性 | Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. Contains 1 protein kinase domain. |
| 功能 | Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates NFATC1/NFATC on conserved serine residues promoting NFATC1/NFATC nuclear export, shutting off NFATC1/NFATC gene regulation, and thereby opposing the action of calcineurin. Phosphorylates MAPT/TAU on 'Thr-548', decreasing significantly MAPT/TAU ability to bind and stabilize microtubules. MAPT/TAU is the principal component of neurofibrillary tangles in Alzheimer disease. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Phosphorylates MACF1, inhibiting its binding to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. Probably regulates NF-kappa-B (NFKB1) at the transcriptional level and is required for the NF-kappa-B-mediated anti-apoptotic response to TNF-alpha (TNF/TNFA). Negatively regulates replication in pancreatic beta-cells, resulting in apoptosis, loss of beta-cells and diabetes. Phosphorylates MUC1 in breast cancer cells, decreasing the interaction of MUC1 with CTNNB1/beta-catenin. Is necessary for the establishment of neuronal polarity and axon outgrowth. Phosphorylates MARK2, leading to inhibit its activity. Phosphorylates SIK1 at 'Thr-182', leading to sustain its activity. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {ICC/IF} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1ug/Test} |
The blue histogram is unstained cells(A549 cells).
The Orange histogram is cells stained with Rabbit IgG/FITC (bs-0295P-FITC).
The green histogram is cells stained with Rabbit Anti-phospho-GSK-3 Beta(Ser9)/FITC Conjugated antibody (bs-2066R-FITC).
Isotype control: Cell lines treated with Rabbit IgG/FITC(bs-0295P-FITC) instead of the primary antibody to confirm that primary antibody binding is specific. Concentration: 5μL in 100 μL 1 X PBS containing 0.5% BSA.
The Orange histogram is cells stained with Rabbit IgG/FITC (bs-0295P-FITC).
The green histogram is cells stained with Rabbit Anti-phospho-GSK-3 Beta(Ser9)/FITC Conjugated antibody (bs-2066R-FITC).
Isotype control: Cell lines treated with Rabbit IgG/FITC(bs-0295P-FITC) instead of the primary antibody to confirm that primary antibody binding is specific. Concentration: 5μL in 100 μL 1 X PBS containing 0.5% BSA.
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-GSK-3 Beta(Ser9) Polyclonal Antibody, Unconjugated(bs-2066R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-GSK-3 Beta(Ser9) Polyclonal Antibody, Unconjugated(bs-2066R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta(Ser9)) Polyclonal Antibody, Unconjugated (bs-2066R p-GSK-3) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-phospho-GSK-3 Beta (Ser9) antibody (bs-2066R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-phospho-GSK-3 Beta (Ser9) antibody (bs-2066R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Tissue/cell:Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-GSK-3 Beta (Ser9)) polyclonal Antibody, Unconjugated (bs-2066R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Primary: Anti-phospho-GSK-3 Beta (Ser9) (bs-2066R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Primary: Anti-phospho-GSK-3 Beta (Ser9) (bs-2066R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
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文献和实验该产品被引用文献
[IF={{ 7.971 }}] {He J et al. SLC34A2 Simultaneously Promotes Papillary Thyroid Carcinoma Growth and Invasion Through Distinct Mechanisms. Oncogene. 2020 Mar;39(13):2658-2675.} {WB} {human}
[IF={{ 6.222 }}] {Wang, Lili. et al. Progranulin improves neural development via the PI3K/Akt/GSK-3β pathway in the cerebellum of a VPA-induced rat model of ASD. Transl Psychiat. 2022 Mar;12(1):1-14} {WB} {Rat}
[IF={{ 6.17 }}] {Tong Xu. et al. Lithium chloride represses abdominal aortic aneurysm via regulating GSK3β/SIRT1/NF-κB signaling pathway. Free Radical Bio Med. 2021 Apr;166:1} {WB,IHC} {Rat}
[IF={{ 6.023 }}] {Ling Xie. et al. Suppression of GOLM1 by EGCG through HGF/HGFR/AKT/GSK-3β/β-catenin/c-Myc signaling pathway inhibits cell migration of MDA-MB-231. Food Chem Toxicol. 2021 Nov;157:112574} {WB} {human}
[IF={{ 5.6 }}] {Jiang Hongyuan. et al. Establishment of a prognostic risk model for osteosarcoma and mechanistic investigation. FRONT PHARMACOL. 2024 Apr;15:} {IF} {Human,Mouse}
相关实验
的关系理不出头绪。GSK-3 beta 总蛋白减少，p-GSK-3 beta (ser 9)增加，是不是足以说明有活性的GSK-3 beta 减少更多，这样理解不知道对不对，希望给以指点。 galaxy22 楼主你好，请问你做出结果的tyr216的抗体是哪个公司的。。。我做出来总量是减少的，active B catenin是减少的，那我有必要做ser9 和tyr216的磷酸化形式吗？我想研究wnt和akt。。。 棉花糖的温柔
of the serine/threonine kinaseAkt, which will phosphorylate GSK-3 at specific serine residues(Ser21 in GSK-3a and Ser9 in GSK-3b), leading to the inactivationof GSK-3 kinase activity (10) . However, this phosphorylationand inactivation of GSK-3 by Akt
beta激酶活性增加，或者说GSK-3β被激活，那么β-catenin应该减少，这样理解对吧？ 对的~ 我是selleckchem中国分公司的技术支持，有关信号转导的问题，以后可以常交流~ zhangyinghua8106 多谢selleckchem01的解疑。但是我看到“GSK-3是PI3 kinase/Akt信号通路的关键下游蛋白，Akt可以磷酸化GSK-3α的Ser21和GSK-3β的Ser9，并抑制它们的酶活
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phospho-GSK-3 Beta (Ser9) Rabbit pAb(bs-2066R)-50ul/100ul/200ul
￥1180 - 2800
