Blank control (blue): U937 cells(fixed with 2% Paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti-CD11c antibody(bs-2508R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
- ￥1180 - 2800
- Bioss
- bs-2508R
- 2025年10月16日
- 产品信息以Bioss网站为准
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-2508R |
| 英文名称 | CD11c Rabbit pAb |
| 中文名称 | 整合素αX抗体 |
| 英文别名 | Integrin alpha-X; integrin alpha-X precursor; ITGAX; SLEB6; CD11C(p150) alpha polypeptide; complement receptor 4; integrin aX; CD11 antigen-like family member C; leukocyte adhesion receptor p150, 95; leukocyte adhesion glycoprotein p150, 95 alpha chain; ITAX_HUMAN. |
| 产品应用 | Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Dog) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Integrin Alpha X |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 126 kDa |
| 实际分子量 | 145-150 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Immunology > Cell Type Markers > CD > Adhesion Signal Transduction > Cytoskeleton / ECM > Cell Adhesion > Integrins > Alpha Stem Cells > Hematopoietic Progenitors > Lymphoid > T Lymphocytic Lineage Stem Cells > Hematopoietic Progenitors > Myeloid > Dendritic Cell Lineage Stem Cells > Hematopoietic Progenitors > Myeloid > Monocytic Lineage |
| 亚细胞定位 | Membrane. |
| 组织特异性 | Predominantly expressed in monocytes and granulocytes. |
| 相似性 | Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats. Contains 1 VWFA domain |
| 功能 | Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. [provided by RefSeq]. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1μg/Test} |
Blank control: Raw264.7.
Primary Antibody (green line): Rabbit Anti-CD11c antibody (bs-2508R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-CD11c antibody (bs-2508R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: Raw264.7.
Primary Antibody (green line): Rabbit Anti-CD11c antibody (bs-2508R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-CD11c antibody (bs-2508R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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文献和实验该产品被引用文献
[IF={{ 7.727 }}] {Jinbo Li. et al. Low dose shikonin and anthracyclines coloaded liposomes induce robust immunogenetic cell death for synergistic chemo-immunotherapy. J Control Release. 2021 Jul;335:306} {IHC} {Mouse}
[IF={{ 7.577 }}] {Wei C et al. Nanocolloids in drinking water increase the risk of obesity in mice by modulating gut microbesEnviron Int.2021 Jan;146:106302.} {IHC} {Mouse}
[IF={{ 7.27 }}] {Zhang, Xinwen, et al. "Peripheral Role of Cathepsin S in Th1 Cell-Dependent Transition of Nerve Injury-Induced Acute Pain to a Chronic Pain State." The Journal of Neuroscience 34.8 (2014): 3013-3022.} {Other} {="Rat"}
[IF={{ 5.8 }}] {Fenghe Du. et al. Imbalance of helper T cell subtypes and adipokine secretion in perivascular adipose tissue as a trigger of atherosclerosis in chronic Porphyromonas gingivalis W83 infection. MICROBES INFECT. 2023 Jul;:105181} {IF} {Mouse}
[IF={{ 5.085 }}] {Xianmei Liu. et al. Tropomodulin1 Expression Increases Upon Maturation in Dendritic Cells and Promotes Their Maturation and Immune Functions. Front Immunol. 2020; 11: 587441} {WB,FCM} {Mouse}
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CD11c Rabbit pAb(bs-2508R)-50ul/100ul/200ul
￥1180 - 2800
