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anti-HSP70

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  • ￥1180 - 2800
  • Bioss已认证
  • 北京
  • bs-0126R
  • 2025年10月15日
  • WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:400-800,IHC-F=1:400-800,Flow Cyt=1μg/Test,IF=1:100-500,ICC=1:100-500,
  • Rabbit
  • Human,Mouse,Rat,Chicken,Dog,Pig,Rabbit,
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北京博奥森生物技术有限公司
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    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 免疫原：

      KLH conjugated synthetic peptide derived from human HSP70

    • 亚型：

      IgG

    • 形态：

      Lyophilized or Liquid

    • 保存条件：

      Store at -20 °C for one year.

    • 克隆性：

      单克隆

    • 标记物：

      无

    • 适应物种：

      Human,Mouse,Rat,Chicken,Dog,Pig,Rabbit,

    • 保质期：

      1年

    • 目录编号：

      bs-0126R

    • 库存：

      999

    • 供应商：

      北京博奥森生物技术有限公司

    • 宿主：

      Rabbit

    • 应用范围：

      WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:400-800,IHC-F=1:400-800,Flow Cyt=1μg/Test,IF=1:100-500,ICC=1:100-500,

    • 浓度：

      1mg/ml

    • 抗体英文名：

      HSP70

    • 抗体名：

      热休克蛋白-70抗体

    • 规格：

      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.00
    规格：100ul产品价格：￥1980.00
    规格：200ul产品价格：￥2800.00
    产品编号bs-0126R
    英文名称HSP70 Rabbit pAb
    中文名称热休克蛋白-70抗体
    英文别名HSP70; HSP-70; HSP 70; Heat shock 70 kDa protein 1; heat shock 70kDa protein 1A; Heat shock 70kDa protein 1B; Heat shock induced protein; heat shock protein 70; HSP70 1; HSP70 2; HSP70.1; HSP72; HSPA1; HSPA1A; HSPA1B; XXbac BCX40G17.3 001; Heat shock 70 kDa protein 1A/1B; HSP71A_HUMAN; Hspa1a; Hsp70-1; HSP72; Hspa1; Hspa1b; Heat shock 70 kDa protein 1A/1B; shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, Flow-Cyt=1ug/Test

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat (Chicken, Cow, Rabbit, Sheep)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human HSP70
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量70 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cancer > Tumor biomarkers

    Signal Transduction > Protein Trafficking > Chaperones > Heat Shock Proteins

    亚基Component of the CatSper complex. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with TSC2. Interacts with IRAK1BP1. Interacts with TERT; the interaction occurs in the absence of the RNA component, TERC, and dissociates once the TERT complex has formed. Interacts with DNAJC7. Interacts with CHCHD3.
    亚细胞定位Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
    组织特异性HSPA1B is testis-specific.
    相似性Belongs to the heat shock protein 70 family.
    功能In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008].

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {ICC/IF}{1:100}
    {IF}{1:100-500}
    {Flow-Cyt}{1ug/Test}

     

    产品细节图片1
    Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-HSP-70 Polyclonal Antibody, Unconjugated(bs-0126R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    产品细节图片2
    Blank control (blue line): Jurkat (blue).
    Primary Antibody (green line): Rabbit Anti-HSP70 antibody (bs-0126R)
    Dilution: 1μg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
    Dilution: 1μg /test.
    Protocol
    The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
    产品细节图片3
    Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HSP70) polyclonal Antibody, Unconjugated (bs-0126R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
    产品细节图片4
    Tissue/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HSP70) polyclonal Antibody, Unconjugated (bs-0126R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
    产品细节图片5
    Sample: Lane 1: Testis (Mouse) Lysate at 40 ug Lane 2: Epididymis (Mouse) Lysate at 40 ug Lane 3: Seminal vesicle (Mouse) Lysate at 40 ug Lane 4: Liver (Mouse) Lysate at 40 ug Lane 5: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 6: Testis (Rat) Lysate at 40 ug Lane 7: Spleen (Rat) Lysate at 40 ug Lane 8: Hela (Human) Cell Lysate at 30 ug Lane 9: Jurkat (Human) Cell Lysate at 30 ug Lane 10: HepG2 (Human) Cell Lysate at 30 ug Lane 11: A549 (Human) Cell Lysate at 30 ug Lane 12: MCF-7 (Human) Cell Lysate at 30 ug Primary: Anti-HSP70 (bs-0126R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 68 kD
    产品细节图片6
    25 ug total protein per lane of various lysates (see on figure) probed with HSP70 polyclonal antibody, unconjugated (bs-0126R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

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    图标文献和实验
    该产品被引用文献

    [IF={{ 4.08 }}] {Wang, Fu‐Xin, et al. "Proteomic analysis of the sea‐island cotton roots infected by wilt pathogen Verticillium dahliae." Proteomics 11.22 (2011): 4296-4309.} {WB} {="Other Species"}

    [IF={{ 4.2 }}] {Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).} {IHC} {="Mouse"}

    [IF={{ 7.78 }}] {Xu,et al.Ultrasensitive electrochemical sensing of Hg2+ based on thymine-Hg2+-thymine interaction and signal amplification of alkaline phosphatase catalyzed silver deposition.(2018) Biosensors & Bioelectronics. 104:95-101.} {Other} {}

    [IF={{ 2.741 }}] {Shao,et al.Arsenic and/or copper caused inflammatory response via activation of inducible nitric oxide synthase pathway and triggered heat shock protein responses in testis tissues of chicken.(2018) Environmental Science and Pollution Research. 25:7719-7729.} {WB} {Chicken}

    [IF={{ 1.652 }}] {Shen H et al. Effects of elevated ambient temperature and local testicular heating on the expressions of heat shock protein 70 and androgen receptor in boar testes. Acta Histochem. 2019 Feb 2. pii: S0065-1281(18)30339-8.} {WB,IHC} {boar}

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    • Nucleotide Exchange Factors for Hsp70 Chaperones

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