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Pan Methylated Lysine Mouse mA

b(bsm-33197M)-50ul/100ul/200ul/200ug
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  • ¥1180 - 5600
  • Bioss已认证
  • bsm-33197M
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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      50ul/100ul/200ul/200ug

    规格:50ul产品价格:¥1180.0
    规格:100ul产品价格:¥1980.0
    规格:200ul产品价格:¥2800.0
    规格:200ug产品价格:¥5600.0
    产品编号bsm-33197M
    英文名称Pan Methylated Lysine Mouse mAb
    中文名称泛甲基化赖氨酸单克隆抗体
    英文别名Dimethylysine; Methyllysine; Trimethyllysine; Dimethy-lysine; Methyl-lysine; Trimethyl-lysine; N epsilon dimethyl lysine; N epsilon mono methyllysine; N epsilon trimethyl lysine; N(epsilon) trimethyl lysine; di methyl-lysine, mono methyl-lysine, Trimethyl-lysine; methyl K pan; di methyl-K, mono methyl-K, Trimethyl-K.
    中文别名泛甲基化赖氨酸; 甲基化修饰赖氨酸;
    产品应用WB=1:1000-5000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-200, IF=1:100-500, Flow-Cyt=1μg/Test

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Species independent
    抗体来源Mouse
    免疫原BSA conjugated Methylated Lysine (di methyl, mono methyl, Trimethyl-lysine)
    亚型IgG1
    性状Liquid
    纯化方法affinity purified by Protein G
    克隆类型Monoclonal
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

     

    应用推荐稀释比例
    {WB}{1:1000-5000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {ICC/IF}{1:100-200}
    {IF}{1:100-500}
    {Flow-Cyt}{1μg/Test}

     

    产品细节图片1
    The Hela (treated with Sodium Butyrate (5mM, 24h)) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-Methylated Lysine antibody (bsm-33187M): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (bs-60296G-FITC): 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
    产品细节图片2
    4% P‌‌araformaldehyde-fixed Hela (treated with Sodium Butyrate (5mM, 24h)) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Methylated Lysine) monoclonal Antibody, unconjugated (bsm-33197M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

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    图标文献和实验
    相关实验
    • 组化染色背景高?没信号?一篇文章带你快速掌握免疫组化!

      组织化学分析。(图 C)使用Anti-Erk1/2 Mouse mAb 鼠单抗进行目的蛋白检测;(图 D) 使用p44/42 MAPK (Erk1/2)Rabbit mAb 兔单抗进行目的蛋白检测。 图 7 免疫组化实验检测Akt表达 注:使用 Anti-Akt (pan) Rabbit mAb 对正常小鼠肝组织进行免疫组织化学分析。(图 E)使用免疫组化试剂盒 M&R HRP/DAB Detection IHC Kit,抗体1:100 稀释;(图F)使用另一种试剂盒,抗体 1:100 稀释。

    • 【求助】谁有兔抗Paxillin和鼠抗phorsphotyrosine

      -Acetylated Lysine Antibody-FITC IBN4010 Acetyl-RhoGDI (K141) Antibody IBN4011 Acetyl-PPIA (K124) Antibody IBN4005 e-Methylated Lysine Antibody IBN4008 Pan e-Trimethylysine Antibody IBN4009 Pan e-Trimethylysine Antibody-FITC IBN4003

    • Intracellular Cytokine Staining Protocol

      . More antibodies are in development; if you do not see an antibody of your choice, please send us an e-mail so we may provide you timely product updates.Table 1: Mouse Cytokine Intracellular Staining Quick Guide Mouse CytokineCell SourceActivationIncubation

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