Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Rat Liver tissue lysates
Lane 3: Human THP-1 cell lysates
Lane 4: Human A431 cell lysates
Primary: Anti-Histone H1.0 (bs-33535R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 32 kDa
- ￥1180 - 1980
- Bioss
- bs-33535R
- 2025年10月16日
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50ul/100ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 产品编号 | bs-33535R |
| 英文名称 | Histone H1.0 Rabbit pAb |
| 中文名称 | 组蛋白H1.0抗体 |
| 英文别名 | H10_HUMAN; H1-0; Histone H1'; Histone H1(0); H1F0; H1FV; H1.0 linker histone; H10; |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Histone H1.0 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 21 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > DNA / RNA > DNA Damage & Repair > Non Homol. End Joining Epigenetics and Nuclear Signaling > Histones > H1 > Unmodified Epigenetics and Nuclear Signaling > Histones > Variants |
| 亚细胞定位 | Cytoplasm. Nucleus. Chromosome. |
| 组织特异性 | Oocyte-specific. |
| 翻译后修饰 | Phosphorylated on Ser-17 in RNA edited version. |
| 相似性 | Belongs to the histone H1/H5 family. Contains 1 H15 (linker histone H1/H5 globular) domain. |
| 功能 | May play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family. [provided by RefSeq, Oct 2015] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {ELISA} | {1:5000-10000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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文献和实验相关实验
】Histone IIIs的电泳现象 请教各位战友，Histone IIIs的分子量是多大啊？在跑SDS/PAGE的时候是不是会形成二聚体？ 谢过！ http://www.dxy.cn/bbs/post/view?bid=65&id=5002861&sty=1&tpg=21&age=0 [求助]关于DEAE层析 请问各位高人，DEAE-MacroPrep column (Bio-Rad)，具体原理是什么？非常感谢。 http://www.dxy.cn/bbs/post/view?bid=65
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Histone H1.0 Rabbit pAb(bs-33535R)-50ul/100ul
￥1180 - 1980
