Sample:
_x000D_
Cerebrum (Mouse) Lysate at 40 ug
_x000D_
Primary: Anti-SH3GLB2 (bs-21173R) at 1/1000 dilution
_x000D_
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_
Predicted band size: 44 kD
_x000D_
Observed band size: 44 kD
- ￥1180 - 2800
- Bioss
- bs-21173R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-21173R |
| 英文名称 | SH3GLB2 Rabbit pAb |
| 中文名称 | 胞浆蛋白SH3GLB2抗体 |
| 英文别名 | Endophilin B2; Endophilin-B2; OTTHUMP00000022320; PP6569; PP9455; Retinoid receptor induced gene 1; RRIG1; SH3 containing protein SH3GLB2; SH3 domain GRB2 like endophilin B2; SH3 domain-containing GRB2-like protein B2; SH3GLB2; SHLB2_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human SH3GLB2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 44 kDa |
| 浓度 | 1mg/ml |
| 储存液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 研究领域 | Cancer > Tumor immunology > Tumor-associated antigens Cell Biology > Other Antibodies > Other Antibodies |
| 亚基 | Homodimer, and heterodimer with SH3GLB1. |
| 亚细胞定位 | Cytoplasm. |
| 组织特异性 | Detected in skeletal muscle, adipocyte, brain, lung, colon and mammary gland. |
| 相似性 | Belongs to the endophilin family. _x000D_ Contains 1 BAR domain. _x000D_ Contains 1 SH3 domain. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | SH3GLB2 (SH3 Domain Containing GRB2 Like, Endophilin B2) is a Protein Coding gene. Among its related pathways are Endocytosis. An important paralog of this gene is SH3GLB1. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SH3GLB2) Polyclonal Antibody, Unconjugated (bs-21173R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining._x000D_
_x000D_
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文献和实验相关实验
, Algermissen, B. et al. 3. Purification of rabbit liver aldehyde oxidase by affinity chromatography on benzamidine Sepharose 6B. J. Chromatogr. (1989), 475 363-72, Stell, J. G. P. et al. 4. The Crystal Structure of a T Cell Receptor in Complex
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
. 7:8‐16. Christie, M.R., Hollands, J.A., Brown, T.J., Michelsen, B.K., and Delovitch, T.L. 1993. Detection of pancreatic islet 64,000 M(r) autoantigens in insulin‐dependent
Models and Analysis of Atherosclerosis, Restenosis, and Aneurysm Formation in the Mouse
Avidin‐biotin complex (ABC) kit (Dako) 3,3′‐diaminobenzidine (Bright DAB solution; ImmunoLogic, cat. no. BS04‐500A/B; http://www.immunologic.nl
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SH3GLB2 | Endophilin-B2 Rabbit pAb(bs-21173R)-50ul/100ul/200ul
￥1180 - 2800
