Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SP-A Polyclonal Antibody, Unconjugated(bs-0770R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
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- bs-0770R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0770R |
| 英文名称 | SFTPA1 Rabbit pAb |
| 中文名称 | 肺表面活性蛋白A抗体 |
| 英文别名 | SP-A; SFTPA2; SFTPA1; Surfactant Protein A; pulmonary surfactant-associated glycoprotein A; Pulmonary surfactant-associated protein A2 precursor; SP-A1; SP-A2; SP-A; PSP-A; PSPA; Alveolar proteinosis protein; 35 kDa pulmonary surfactant-associated protein; MGC133365; Pulmonary surfactant apoprotein; Pulmonary surfactant associated protein; SFTP1; SFTPA; SFTPA1B; SP A; SP A1; Surfactant pulmonary associated protein A1; PSPA; SPA2; SFTP1; SPAII; COLEC5; SFTPA2B; SFTA1_HUMAN; SFPA2_HUMAN; Pulmonary surfactant-associated protein A1; Pulmonary surfactant-associated protein A2. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human SFTPA2/SFTPA1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 25 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Cytoskeleton / ECM > Extracellular Matrix > ECM Proteins > Other ECM Proteins |
| 亚基 | Oligomeric complex of 6 set of homotrimers. |
| 亚细胞定位 | Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film. |
| 相似性 | Belongs to the SFTPA family.
Contains 1 C-type lectin domain. Contains 1 collagen-like domain. |
| 功能 | In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene is one of several genes encoding pulmonary-surfactant associated proteins (SFTPA) located on chromosome 10. Mutations in this gene and a highly similar gene located nearby, which affect the highly conserved carbohydrate recognition domain, are associated with idiopathic pulmonary fibrosis. The current version of the assembly displays only a single centromeric SFTPA gene pair rather than the two gene pairs shown in the previous assembly which were thought to have resulted from a duplication. [provided by RefSeq, Sep 2009] |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SP-A Polyclonal Antibody, Unconjugated(bs-0770R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SP-A Polyclonal Antibody, Unconjugated(bs-0770R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Formalin-fixed and paraffin embedded mouse lung tissue labeled Anti-SP-A Polyclonal Antibody, Unconjugated (bs-0770R) at 1:2000, followed by conjugation to the secondary antibody and DAB staining
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文献和实验该产品被引用文献
[IF={{ 3.061 }}] {Fang Q et al. pre-B cell colony enhancing factor negatively regulates Na and fluid transport in lung epithelial cells. Am J Transl Res. 2018 Jul 15;10(7):2047-2054. eCollection 2018.} {ICC,WB} {Rat}
[IF={{ 2.741 }}] {Tara Bocking. et al. Research article expression of surfactant protein-A and D, and CD9 in lungs of 1 and 30 day old foals. Bmc Vet Res. 2021 Dec;17(1):1-9} {WB,IHC} {Foal}
[IF={{ 0.267 }}] {Tai-Wen Raoet al. Effect of oxygen supplement during targeted temperature management on acute lung injury in the early stage of traumatic hemorrhagic shock. European Journal of Inflammation Volume 18: 1–7} {WB} {rabbit}
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SFTPA1 Rabbit pAb(bs-0770R)-50ul/100ul/200ul
￥1180 - 2800
