Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-Placental alkaline phosphatase (bs-20015R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
- ￥1180 - 2800
- Bioss
- bs-20015R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-20015R |
| 英文名称 | Placental alkaline phosphatase (PLAP) Rabbit pAb |
| 中文名称 | 碱性磷酸酶(胎盘型)抗体 |
| 英文别名 | Alkaline phosphatase; Alkaline phosphatase placental; Alkaline phosphatase placental type; Alkaline phosphatase Regan isozyme; ALP; Alp1; ALPP; FLJ61142; Germ-cell alkaline phosphatase; nagao isozyme; OTTHUMP00000164354; PALP; Placental alkaline phosphatase 1; placental heat-stable alkaline phosphatase; placental type; PLAP; PLAP-1; PLAP1; PPB1_HUMAN. |
| 中文别名 | 碱性磷酸酶胎盘型; 胎盘碱性磷酸酶; |
| 产品应用 | WB=1:500-2000, Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Placental alkaline phosphatase 1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 53 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Tags & Cell Markers > Fusion / Marker Proteins > Alkaline Phosphatase |
| 亚基 | Homodimer. |
| 亚细胞定位 | Cell membrane; Lipid-anchor, GPI-anchor. |
| 组织特异性 | Trace amounts in the testis and thymus, and in elevated amounts in germ cell tumors. |
| 相似性 | Belongs to the alkaline phosphatase family. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {1μg/Test} |
Blank control (blue line): Hep G2(fixed with 70% ethanol Overnight at 4℃).
Primary Antibody (green line): Rabbit Anti-Placental alkaline phosphatase antibody (bs-20015R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1μg /test.
Primary Antibody (green line): Rabbit Anti-Placental alkaline phosphatase antibody (bs-20015R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1μg /test.
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文献和实验相关实验
单座位复等位基因同工酶是指同一座位上的不同等位基因所编码的酶蛋白。例如胎盘碱性磷酸酶（placental alkaline phosphatase,PLAP）是一种典型的复等位基因编码的酶，PLAP为二聚体，基因定位于2q37，依电泳速度可分3种变异型即慢（S）、中（I）、快（F）型，它们受3个复等位基因PLs、PLi及PLf控制，每种基因分别编码S、I、F三种同工酶（G6PD）和腺苷酸激酸（AK）的同工酶即属此类。
in conjunction with X-Phos as the final electron acceptor for the indoxyl dimerization reaction. When reduced, NBT forms a purple precipitate. Of the cloned phosphatases, the human placental alkaline phosphatase gene, PLAP (Kam et al 1985
LINEAGE ANALYSIS USING RETROVIRAL VECTORS
when choosing a vector for lineage analysis are the reporter gene and the promoter that drives its expression. The reporters that have been used include cytoplasmic lacZ [18], nuclear lacZ [19], human placental alkaline phosphatase (PLAP) [20], and avian gag [21
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Placental alkaline phosphatase (PLAP) Rabbit pAb(bs-20015R)-50ul/100ul/200ul
￥1180 - 2800
