Sample:Heart (Mouse) Lysate at 40 ug
_x000D_
Primary: Anti-MAPKAPK2/3 (bs-18674R) at 1/300 dilution
_x000D_
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_
Predicted band size: 46 kD
_x000D_
Observed band size: 46 kD
_x000D_
- ￥1180 - 2800
- Bioss
- bs-18674R
- 2026年10月13日
企业认证
相关产品推荐更多 >
COX7C Rabbit pAb, PE conjugated(bs-14016R-PE)-100ul
￥2980
H2BW2 | Histone H2B.s Rabbit pAb(bs-15400R)-50ul/100ul/200ul
￥1180
DDX18 Rabbit pAb(bs-14216R)-50ul/100ul/200ul
￥1180NFKBIA | IκB alpha (p-S32/S36) Rabbit pAb(bs-2513R)-50ul/100ul/200ul
￥1180ATG9B | APG9L2 Rabbit pAb(bs-4011R)-50ul/100ul/200ul
￥1180
万千商家帮你免费找货
0 人在求购买到急需产品
相关阅读
香烟烟雾如何加速声带纤维化？CD44-透明质酸轴是关键通路Takara 秋促来袭，提取+反转录+定量套装仅 1200 元五大应用案例：Mustang Q 膜层析应用全解析1 个小工具，一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-18674R |
| 英文名称 | MAPKAPK2/3 Rabbit pAb |
| 中文名称 | 丝裂原活化蛋白激酶活化的蛋白激酶2/3抗体 |
| 英文别名 | 3PK; AA960234; MAP kinase activated protein kinase 2; MAP kinase activated protein kinase 3; MAPK activated protein kinase 2; MAPK activated protein kinase 3; MAPKAP kinase 2; MAPKAP kinase 3; MK2; MK3; OTTHUMP00000034531; Rps6kc1. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human MAPKAPK2/3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 46 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚细胞定位 | Cytoplasmic and Nuclear |
| 组织特异性 | Expressed in all tissues examined. |
| 翻译后修饰 | Phosphorylated and activated by MAPK1/ERK2 and MAPK3/ERK1. Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-201, Ser-251 and Thr-313. |
| 相似性 | Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family._x000D_ Contains 1 protein kinase domain. |
| 功能 | Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
Sample: Muscle (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-MAPKAPK2/3 (bs-18674R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 46 kD
_x000D_ Observed band size: 46 kD
_x000D_
_x000D_ Primary: Anti-MAPKAPK2/3 (bs-18674R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 46 kD
_x000D_ Observed band size: 46 kD
_x000D_
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
Steady State ATPase Assays Coupled Enzyme System
DDW 10 mM Mg・ATP 30 mM PEP 6 mM NADH (Make fresh in 10 mM Tris-OAc pH 7.5, 4.26 mg/mL = 6 mM) PK/LDH (Sigma #P-0294, PK + LDH
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
Immunostaining 24. Wash stored slides twice in PBS (10 min each wash). 25. Wash slides once in PBS containing 1% Triton X-100 for 10 min. 26. Incubate slides in a slide jar containing blocking solution for 1 h
技术资料暂无技术资料 索取技术资料
MAPKAPK2&MAPKAPK3 Rabbit pAb(bs-18674R)-50ul/100ul/200ul
￥1180 - 2800
