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- bs-0331R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0331R |
| 英文名称 | Hepatitis B Virus Surface Antigen Rabbit pAb |
| 中文名称 | 肝炎病毒S蛋白抗体 |
| 英文别名 | HBSAG_HBVE1; Large envelope protein; L glycoprotein; L-HBsAg; LHB; Large S protein; Large surface protein; Major surface antigen. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | (Hepatitis B virus (HBVE1)) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from HBVE1 Hepatitis B Virus Surface Antigen |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 44 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Microbiology > Organism > Virus > DNA Virus > double stranded DNA Virus > Hepatitis B |
| 亚基 | Li-HBsAg interacts with capsid protein and with HDV Large delta antigen. Isoform M associates with host chaperone CANX through its pre-S2 N glycan. This association may be essential for M proper secretion. |
| 亚细胞定位 | Virion membrane. |
| 翻译后修饰 | Isoform M is N-terminaly acetylated at a ratio of 90%, and N-glycosylated at the pre-S2 region.
Myristoylated. |
| 相似性 | Belongs to the orthohepadnavirus major surface antigen family. |
| 功能 | The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assumes fusion between virion membrane and endosomal membrane (Probable). In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein.
The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {ICC/IF} | {1:100-500} |
| {IF} | {1:100-500} |
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文献和实验Processing of Hepatitis B Virus Surface Proteins
The hepatitis B virus (HBV) can cause acute as well as chronic infections of the human liver. More than 300 million individuals are chronically infected worldwide, and it has been estimated that about 1 million of them die annually
Detection of Hepatitis B Virus X Antigen by Immunohistochemistry and Western Blotting
The genome of hepatitis B virus (HBV) is a partially double-stranded DNA molecule within virus particles that is approx 3.2 kbp long (1 ,2 ). It has four open reading frames, all on one strand, that encode the surface antigen or envelope
Transcriptional Control of Hepatitis B Virus
Hepatitis B virus (HBV) transcriptional regulation has been extensively studied in vitro by transient transfection using the activity of a reporter molecule as the readout for transcriptional activity (1 –5 ). Several reporter molecules
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