Blank control: Molt4.
Primary Antibody (green line): Rabbit Anti- FMNL3/FITC Conjugated antibody (bs-16151R-FITC)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-FITC .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-16151R
- 2025年10月16日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-16151R |
| 英文名称 | FMNL3 Rabbit pAb |
| 中文名称 | FMNL3蛋白抗体 |
| 英文别名 | FHOD3; FMNL3_HUMAN; Formin homology 2 domain containing protein 3; Formin like protein 3; WBP3; WW domain binding protein 3. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Dog, Cow) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human FMNL3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 117 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microfilaments > Actin etc > Actin Binding Proteins |
| 亚基 | Interacts with SRGAP2 (via SH3 domain). |
| 亚细胞定位 | Cytoplasm. Note=Enriched in lamellipodia. |
| 相似性 | Belongs to the formin homology family. Contains 1 DAD (diaphanous autoregulatory) domain. Contains 1 FH2 (formin homology 2) domain. Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain. |
| 功能 | FMNL3 is an actin-binding protein that contains a formin homology 2 domain. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene contains a formin homology 2 domain and has high sequence identity to the mouse Wbp3 protein. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1ug/test} |
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FMNL3) Polyclonal Antibody, Unconjugated (bs-16151R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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文献和实验该产品被引用文献
[IF={{ 4.966 }}] {Pan ZN et al. RAB7 GTPase regulates actin dynamics for DRP1‐mediated mitochondria function and spindle migration in Mouseoocyte meiosis. FASEB J. 2020 Jul;34(7):9615-9627.} {IP} {Mouse}
[IF={{ 4.966 }}] {Pan Z et al. RAB7 GTPase regulates actin dynamics for DRP1‐mediated mitochondria function and spindle migration in Mouseoocyte meiosis. FASEB J . 2020 Jul;34(7):9615-9627.} {WB} {Mouse}
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FMNL3 Rabbit pAb(bs-16151R)-50ul/100ul/200ul
￥1180 - 2800
