Tissue/cell: human breast cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GPR91 Polyclonal Antibody, Unconjugated(bs-15392R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
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- bs-15392R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-15392R |
| 英文名称 | GPR91 Rabbit pAb |
| 中文名称 | G蛋白偶联受体GPR91蛋白抗体 |
| 英文别名 | G protein coupled receptor 91; G-protein coupled receptor 91; GPR 91; P2Y purinoceptor 1; P2Y purinoceptor 1-like; Purinergic Receptor P2U2; Succinate receptor; Succinate receptor 1; SUCN R1; SUCNR 1; SUCNR1; SUCR1_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GPR91 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 39 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Signaling Pathway > G Protein Signaling > GPCR Tags & Cell Markers > Cell Type Markers > Other Cell Types |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. |
| 组织特异性 | Expressed specifically in kidney. |
| 相似性 | Belongs to the G-protein coupled receptor 1 family. |
| 功能 | Receptor for succinate. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a G-protein-coupled receptor for succinate, an intermediate molecule of the citric acid cycle. It is involved in the promotion of hematopoietic progenitor cell development, and it has a potential role in renovascular hypertension which has known correlations to renal failure, diabetes and atherosclerosis. [provided by RefSeq, Oct 2009] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Western blot analysis of extracts from Liver tissue(mo) using GPR91 Antibody.
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GPR91 Polyclonal Antibody, Unconjugated(bs-15392R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GPR91 Polyclonal Antibody, Unconjugated(bs-15392R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Protein: 1.kidney lyates(mo);2.liver lyates(mo);
Primary: Rabbit Anti-GPR91 (bs-15392R) at 1:300;
Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000;
Predicted band size:39 kD Observed band size:49 kD
Primary: Rabbit Anti-GPR91 (bs-15392R) at 1:300;
Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000;
Predicted band size:39 kD Observed band size:49 kD
Sample: HepG2 Cell (Human) Lysate at 40 ug
Primary: Anti-GPR91(bs-15392R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39kD
Observed band size: 39kD
Primary: Anti-GPR91(bs-15392R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39kD
Observed band size: 39kD
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GPR91 Rabbit pAb(bs-15392R)-50ul/100ul/200ul
￥1180 - 2800
