Sample: Hela Cell (Human) Lysate at 40 ug
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Primary: Anti-C21orf33 (bs-15124R) at 1/300 dilution
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Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
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Predicted band size: 28 kD
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Observed band size: 28 kD
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- ￥1180 - 2800
- Bioss
- bs-15124R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-15124R |
| 英文名称 | C21orf33 Rabbit pAb |
| 中文名称 | 21号染色体开放阅读框33抗体 |
| 英文别名 | Chromosome 21 open reading frame 33; D21S2048E; ES 1; ES1; Es1 protein; ES1 protein homolog mitochondrial; GT335; HES 1; HES1; Human HES1 protein homolog to E.coli and zebrafish ES1 protein; Keio novel protein I; KNP I; KNPH; KNPI; Protein GT335; Protein KNP I; ES1_HUMAN; GATD3A |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse, Chicken, Dog, Pig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human C21orf33 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 28 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1μg/Test} |
Blank control: hela(blue), the cells were fixed with 2% Paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice..
_x000D_ Isotype Control Antibody: Rabbit IgG(orange) ; _x000D_ Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), _x000D_ Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; _x000D_ Primary Antibody Dilution: 1ug in 100 uL1X PBS containing 0.5% BSA(green)._x000D_
_x000D_ Isotype Control Antibody: Rabbit IgG(orange) ; _x000D_ Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), _x000D_ Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; _x000D_ Primary Antibody Dilution: 1ug in 100 uL1X PBS containing 0.5% BSA(green)._x000D_
Tissue/cell: Rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
_x000D_ Incubation: Anti-C21orf33 Polyclonal Antibody, Unconjugated(bs-15124R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
_x000D_ Incubation: Anti-C21orf33 Polyclonal Antibody, Unconjugated(bs-15124R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
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文献和实验该产品被引用文献
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GATD3 | C21orf33 Rabbit pAb(bs-15124R)-50ul/100ul/200ul
￥1180 - 2800
