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- 技术资料
- 应用范围:
产品信息以Bioss网站为准
- 规格:
50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-6736R |
| 英文名称 | SOX11 Rabbit pAb |
| 中文名称 | 转录因子SOX-11蛋白抗体 |
| 英文别名 | SRY(sex determining region Y) box 11; SRY related HMG box gene 11; Transcription factor SOX-11; SOX11_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Pig, Cow, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human SOX11 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 47 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Tumor biomarkers Epigenetics and Nuclear Signaling > Transcription > Domain Families > Developmental Families Epigenetics and Nuclear Signaling > Transcription > Domain Families > HMG Box Epigenetics and Nuclear Signaling > Transcription > Transcription Factors Neuroscience > Neurology process > Neurogenesis Neuroscience > Neurology process > Neuroregeneration > Neuroregeneration Stem Cells > Neural Stem Cells > Intracellular |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Mainly expressed in the developing central nervous system. |
| 相似性 | Contains 1 HMG box DNA-binding domain. |
| 功能 | Transcriptional activator. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. The protein may function in the developing nervous system and play a role in tumorigenesis. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |

Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOX11) Polyclonal Antibody, Unconjugated (bs-6736R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

25 ug total protein per lane of various lysates (see on figure) probed with SOX11 polyclonal antibody, unconjugated (bs-6736R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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SOX11 Rabbit pAb(bs-6736R)-50ul/100ul/200ul
¥1180 - 2800





