Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-RGS2 Polyclonal Antibody, Unconjugated(bs-0492R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-0492R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0492R |
| 英文名称 | RGS2 Rabbit pAb |
| 中文名称 | G蛋白信号转导调节因子2抗体 |
| 英文别名 | Basic helix-loop-helix phosphoprotein G0S8; Cell growth inhibiting protein 31; Cell growth-inhibiting gene 31 protein; G0 to G1 switch regulatory 8 24kD; G0/G1 switch regulatory protein 8; GOS8; OTTHUMP00000060765; Regulator of G protein signaling 2; Regulator of G protein signalling 2 24kD; Regulators of G protein signaling XRGSVI; RGS 2. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse, Chicken, Pig, Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human RGS2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 24 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Atherosclerosis > Hypertension > Vascular remodelling Cardiovascular > Blood > Blood Pressure regulation Cardiovascular > Heart > Hypertrophy Epigenetics and Nuclear Signaling > Transcription > Domain Families > HLH / Leucine Zipper > HLH Signal Transduction > Signaling Pathway > G Protein Signaling > Small G Proteins > Regulators |
| 亚基 | Interacts with EIF2B5. Interacts with PRKG1 (isoform alpha). |
| 亚细胞定位 | Isoform 1: Cell membrane. Cytoplasm. Nucleus, nucleolus.
Isoform 2: Cell membrane. Cytoplasm. Nucleus, nucleolus. Isoform 3: Cell membrane. Cytoplasm. Nucleus, nucleolus. Isoform 4: Cell membrane. Mitochondrion. |
| 组织特异性 | Expressed in acute myelogenous leukemia (AML) and in acute lymphoblastic leukemia (ALL). |
| 翻译后修饰 | Phosphorylated by protein kinase C. Phosphorylation by PRKG1 leads to activation of RGS2 activity. |
| 相似性 | Contains 1 RGS domain. |
| 功能 | Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. May play a role in leukemogenesis. Plays a role in negative feedback control pathway for adenylyl cyclase signaling. Binds EIF2B5 and blocks its activity, thereby inhibiting the translation of mRNA into protein. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 2 belongs to this family. The protein acts as a mediator of myeloid differentiation and may play a role in leukemogenesis. [provided by RefSeq]. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-RGS2 Polyclonal Antibody, Unconjugated(bs-0492R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-RGS2 Polyclonal Antibody, Unconjugated(bs-0492R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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RGS2 Rabbit pAb(bs-0492R)-50ul/100ul/200ul
￥1180 - 2800
