Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-Caveolin-3 (bs-3610R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 17 kD
- ￥1180 - 2800
- Bioss
- bs-3610R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3610R |
| 英文名称 | Caveolin-3 Rabbit pAb |
| 中文名称 | 细胞质膜微囊蛋白-3抗体 |
| 英文别名 | CAV 3; CAV3; Caveolin3; Caveolin 3; LGMD1C; M caveolin; VIP 21; VIP21; AI385751; Cav-3; caveolin-3; M-cav; M-caveolin; CAV3_MOUSE. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=1μg /test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Dog, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from mouse Caveolin-3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 17 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Heart > Cardiac metabolism Cardiovascular > Heart > Hypertrophy Developmental Biology > Organogenesis > Skeletal development > Muscle Signal Transduction > Metabolism > Plasma Membrane > Channels Signal Transduction > Metabolism > Vitamins / Minerals Signal Transduction > Protein Trafficking > Golgi Proteins |
| 亚基 | Homooligomer (By similarity). Interacts with DYSF. Interacts with DLG1 and KCNA5; forms a ternary complex. Interacts with DAG1 (via its C-terminal); the interaction prevents binding of DAG1 with DMD (By similarity). Interacts with TRIM72. Interacts with MUSK; may regulate MUSK signaling. |
| 亚细胞定位 | Golgi apparatus membrane; Peripheral membrane protein (By similarity). Cell membrane; Peripheral membrane protein (By similarity). Membrane, caveola; Peripheral membrane protein (By similarity). Note=Potential hairpin-like structure in the membrane. Membrane protein of caveolae (By similarity). |
| 组织特异性 | Expressed predominantly in muscle. |
| 翻译后修饰 | Sumoylation with SUMO3 by PIAS4 may reduce agonist-induced internalization and desensitization of adrenergic receptor ABRD2 (By similarity). |
| 相似性 | Belongs to the caveolin family. |
| 功能 | May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. May also regulate voltage-gated potassium channels. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Caveolae are specialized domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin multigene family which are integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissue types. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {1μg /test} |
Blank control (blue line):A431(blue).
Primary Antibody (green line): Rabbit Anti-Caveolin-3 antibody(bs-3610R); Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC;Dilution: 1μg /test.
Protocol
The cells were fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature (The cells were fixed with 2% Paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice.). Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-Caveolin-3 antibody(bs-3610R); Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC;Dilution: 1μg /test.
Protocol
The cells were fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature (The cells were fixed with 2% Paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice.). Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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文献和实验相关实验
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GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
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Caveolin-3 Rabbit pAb(bs-3610R)-50ul/100ul/200ul
￥1180 - 2800
