ELYS Rabbit pAb(bs-9880R)-50ul/100ul/200ul

ELYS Rabbit pAb(bs-9880R)-50ul

/100ul/200ul
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  • ￥1180 - 2800
  • Bioss已认证
  • bs-9880R
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-9880R
    英文名称ELYS Rabbit pAb
    中文名称转录因子ELYS蛋白抗体
    英文别名ahctf1; AT hook containing transcription factor 1; DKFZp434N093; ELYS; AT-hook-containing transcription factor 1; ELYS transcription factor like protein TMBS62; ELYS_HUMAN; Embryonic large molecule derived from yolk sac; MST108; MSTP108; Protein ELYS; Protein MEL-28; Putative AT-hook-containing transcription factor 1; TMBS62.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Mouse (Human, Rat, Pig, Cow, Horse, Sheep)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human ELYS
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量252 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cardiovascular > Blood

    Epigenetics and Nuclear Signaling > Transcription > Other factors

    Epigenetics and Nuclear Signaling > Transcription > Transcription Factors

    Stem Cells > Hematopoietic Progenitors > Intracellular Molecules

    亚基Associates with the Nup107-160 subcomplex of the NPC.
    亚细胞定位Cytoplasm. Nucleus matrix. Chromosome; centromere; kinetochore. Nucleus; nucleoplasm. Nucleus; nuclear pore complex. Localizes to the nuclear pore complex (NPC) throughout interphase. Localizes to the kinetochore from prophase, and this appears to require the Nup107-160 subcomplex of the NPC. Localizes to the periphery of chromatin from late anaphase.
    相似性Belongs to the ELYS family.
    Contains 1 A.T hook DNA-binding domain.
    功能 Required for the assembly of a functional nuclear pore complex (NPC) on the surface of chromosomes as nuclei form at the end of mitosis. May initiate NPC assembly by binding to chromatin and recruiting the Nup107-160 subcomplex of the NPC. Also required for the localization of the Nup107-160 subcomplex of the NPC to the kinetochore during mitosis and for the completion of cytokinesis.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料ELYS is a protein of the nuclear matrix that contains one AT hook DNA-binding domain. ELYS is a dual nucleoporin/kinetochore protein required for nuclear pore assembly and proper cell division. Nuclear pores span the nuclear envelope and act as gated aqueous channels to regulate the transport of macromolecules between the nucleus and cytoplasm, from individual proteins and RNAs to entire viral genomes. The A.T. hook near the C-terminus of likely indicates ELYS can function as a transcription factor which may play a specific role in hematopoietic tissues. It also may play a role in early embryo development, as ELYS is critical for the survival of inner cells of the blastocyst.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    ELYS Rabbit pAb(bs-9880R)-50ul
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ELYS) Polyclonal Antibody, Unconjugated (bs-9880R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    ELYS Rabbit pAb(bs-9880R)-50ul
    Sample:
    Spinal cord (Mouse) Lysate at 40 ug
    Primary: Anti- ELYS (bs-9880R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 252 kD
    Observed band size: 252 kD

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    ELYS Rabbit pAb(bs-9880R)-50ul/100ul/200ul
    ￥1180 - 2800