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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-4016R |
| 英文名称 | Histone H4 (Acetyl K8) Rabbit pAb |
| 中文名称 | 乙酰化组蛋白H4抗体 |
| 英文别名 | Histone H4(Acetyl K8); Histone H4(Acetyl Lys8); Acetyl-Histone H4(Lys8); Acetyl-Histone H4(K8); H4F2; H4FN; HIST1H4; HIST2H4; HISTH4H4; methyl histone H4; histone H4; H4_HUMAN; Osteogenic growth peptide; OGP. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Dog, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised acetylpeptide derived from human Histone H4 around the acetylation site of Lys8 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 11 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > ChIP assays > ChIP antibodies Epigenetics and Nuclear Signaling > Histones > H4 > Acetylated |
| 亚基 | The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. |
| 亚细胞定位 | Nucleus. Chromosome. |
| 翻译后修饰 | Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin. Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation. |
| 相似性 | Belongs to the histone H4 family. |
| 功能 | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {ICC/IF} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1ug/test} |
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Histone H4 (Acetyl K8)) polyclonal Antibody, Unconjugated (bs-4016R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (Rat skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H4 (Acetyl K8)) Polyclonal Antibody, Unconjugated (bs-4016R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H4 (Acetyl K8)) Polyclonal Antibody, Unconjugated (bs-4016R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Sample:
Lane 1: Human Hela cell lysates
Lane 2: Human Jurkat cell lysates
Lane 3: Human HL60 cell lysates
Lane 4: Human MCF-7 cell lysates
Primary: Anti-Histone H4 (Acetyl K8) (bs-4016R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 11 kD
Observed band size: 11 kD
Lane 1: Human Hela cell lysates
Lane 2: Human Jurkat cell lysates
Lane 3: Human HL60 cell lysates
Lane 4: Human MCF-7 cell lysates
Primary: Anti-Histone H4 (Acetyl K8) (bs-4016R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 11 kD
Observed band size: 11 kD
Blank control（black line）:NIH/3T3.
Primary Antibody (green line): Rabbit Anti-Histone H4 (Acetyl K8) antibody (bs-4016R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-Histone H4 (Acetyl K8) antibody (bs-4016R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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文献和实验相关实验
Histone Acetylation/Deacetylation As a Regulator of Cell Cycle Gene Expression
-diphosphate (ADP)-ribosylation, methylation, ubiquitination, and acetylation (1 ,2 ). Histone acetylation involves the transfer of an acetyl group from acetyl-coenzyme A (CoA) to the ε-amino group of lysine side chains within the substrate by histone acetyl
) AntibodyRabbitW IHC IC9671Acetyl-Histone H3 (Lys9) AntibodyRabbitW IP IHC IC2591Acetyl-Histone H4 (Lys12) AntibodyRabbitW IP IHC IC2594Acetyl-Histone H4 (Lys8) AntibodyRabbitW IP IHC IC有时组蛋白同时乙酰化和磷酸化修饰，进行转录的调控和活性的调节，因此CST也推出了能同时检测乙酰化和磷酸化的组蛋白的抗体。该系列抗体只识别同时乙酰化和磷酸
结果分析的重要步骤和Trouble shooting的依据，因为非特异性的免疫沉淀难以避免。 一般的设置方法 1 未经免疫 沉淀的超声处理后样本( INPUT)。 2 加特异抗体来源动物的正常血清或无关抗体(MOCK)。 3 阳性对照引物的应用。 4 选择不与目的蛋白质结合的基因组 区域作对照。 如 阳性对照抗体 (Anti-acetyl-Histone H3) * 阴性对照抗体 (Normal Rabbit
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Histone H4 (Acetyl K8) Rabbit pAb(bs-4016R)-50ul/100ul/200ul
￥1180 - 2800
