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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-5006R |
| 英文名称 | PGAM1 Rabbit pAb |
| 中文名称 | 磷酸变位酶1抗体 |
| 英文别名 | BPG dependent PGAM 1; BPG dependent PGAM1; BPG-dependent PGAM 1; OTTHUMP00000059414; PGAM 1; PGAM A; PGAM B; PGAM-B; PGAM1_HUMAN; PGAMA; PGAMB; phosphoglycerate mutase 1; phosphoglycerate mutase 1 brain; phosphoglycerate mutase A; phosphoglycerate mutase A nonmuscle form; phosphoglycerate mutase isozyme B. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Dog, Pig, Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human PGAM2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 29 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Metabolism of carbohydrates Metabolism > Pathways and Processes > Metabolic signaling pathways > Carbohydrate metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Energy Metabolism Signal Transduction > Metabolism > Energy Metabolism |
| 亚基 | Homodimer. |
| 组织特异性 | In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues. |
| 翻译后修饰 | Acetylated at Lys-253, Lys-253 and Lys-254 under high glucose condition. Acetylation increases catalytic activity. Under glucose restriction SIRT1 levels dramatically increase and it deacetylates the enzyme. |
| 相似性 | Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily. |
| 功能 | Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | PGAM1 belongs to the phosphoglycerate mutase family, BPG-dependent PGAM subfamily. It is involved in the interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
_x000D_ Stomach (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-PGAM1 (bs-5006R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 29 kD
_x000D_ Observed band size: 29 kD
_x000D_
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文献和实验, Algermissen, B. et al. 3. Purification of rabbit liver aldehyde oxidase by affinity chromatography on benzamidine Sepharose 6B. J. Chromatogr. (1989), 475 363-72, Stell, J. G. P. et al. 4. The Crystal Structure of a T Cell Receptor in Complex
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
. 7:8‐16. Christie, M.R., Hollands, J.A., Brown, T.J., Michelsen, B.K., and Delovitch, T.L. 1993. Detection of pancreatic islet 64,000 M(r) autoantigens in insulin‐dependent
Models and Analysis of Atherosclerosis, Restenosis, and Aneurysm Formation in the Mouse
Avidin‐biotin complex (ABC) kit (Dako) 3,3′‐diaminobenzidine (Bright DAB solution; ImmunoLogic, cat. no. BS04‐500A/B; http://www.immunologic.nl
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