Sample: Heart (Mouse) Lysate at 40 ug
_x000b_
Primary: Anti- Phospho-PFKFB3/PFK2 (Ser467) (bs-3331R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD
- ￥1180 - 2800
- Bioss
- bs-3331R
- 2025年10月16日
- 产品信息以Bioss网站为准
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3331R |
| 英文名称 | phospho-PFKFB3/PFK2 (Ser467) Rabbit pAb |
| 中文名称 | 磷酸化果糖-2,6-二磷酸酶3/磷酸果糖激酶2抗体 |
| 英文别名 | 6 phosphofructo 2 kinase/fructose 2, 6 bisphosphatase; 6 phosphofructo 2 kinase/fructose 2, 6 biphosphatase 3; 6PF 2 K/Fru 2, 6 P2ASE brain/placenta type isozyme; 6PF 2-K/Fru 2, 6 P2ase 3; fructose 6 phosphate, 2 kinase/fructose 2, 6 bisphosphatase; Inducible 6 phosphofructo 2 kinase/fructose 2, 6 bisphosphatase; iPFK 2; IPFK2; PFK/FBPase 3; PFK-2; PFK2; Renal carcinoma antigen NY REN 56; uPFK 2; PFKFB3; F263_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Chicken, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthesised phosphopeptide derived from human PFK2 around the phosphorylation site of Ser467 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 60 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Metabolism of carbohydrates Cardiovascular > Hypoxia > Hypoxia-Regulated Metabolism > Pathways and Processes > Metabolic signaling pathways > Carbohydrate metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Energy Metabolism Metabolism > Pathways and Processes > Metabolism processes > Hypoxia Neuroscience > Neurology process > Metabolism Signal Transduction > Metabolism > Energy Metabolism |
| 组织特异性 | Ubiquitous. |
| 翻译后修饰 | Phosphorylation by AMPK stimulates activity. |
| 相似性 | In the C-terminal section; belongs to the phosphoglycerate mutase family. |
| 功能 | Synthesis and degradation of fructose 2,6-bisphosphate. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-PFKFB3/PFK2 (Ser467)) Polyclonal Antibody, Unconjugated (bs-3331R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-PFKFB3/PFK2 (Ser467)) Polyclonal Antibody, Unconjugated (bs-3331R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-PFKFB3 PFK2 (Ser467)) Polyclonal Antibody, Unconjugated (bs-3331R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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文献和实验该产品被引用文献
[IF={{ 7.7 }}] {Shintaro Watanuki. et al. Context-dependent modification of PFKFB3 in hematopoietic stem cells promotes anaerobic glycolysis and ensures stress hematopoiesis. ELIFE. 2024 Apr} {ICC} {Mouse}
[IF={{ 4.6 }}] {Fu, Wen, et al. "Bioenergetic mechanisms in astrocytes may contribute to amyloid plaque deposition and toxicity." Journal of Biological Chemistry (2015): jbc-M114.} {Other} {="Human"}
[IF={{ 4.129 }}] {Jiang, Bin. et al. Role of Proximal Intestinal Glucose Sensing and Metabolism in the Blood Glucose Control in Type 2 Diabetic Rats After Duodenal Jejunal Bypass Surgery. Obes Surg. 2022 Jan;:1-11} {WB} {Rat}
[IF={{ 3.546 }}] {Yi,et al.The EMT-related transcription factor snail up-regulates FAPα in malignant melanoma cells.(2018) Experimental Cell Research. 364:160-167.} {IHC} {Human}
[IF={{ 3.231 }}] {Lifang Li. et al. Changes in the Expression of MIF and Other Key Enzymes of Energy Metabolism in the Myocardia of Broiler Chickens with Ascites Syndrome. ANIMALS. 2022 Jan;12(19):2488} {WB} {Chicken}
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phospho-PFKFB3/PFK2 (Ser467) Rabbit pAb(bs-3331R)-50ul/100ul/200ul
￥1180 - 2800
