Blank control:HL-60.
Primary Antibody (green line): Rabbit Anti-Beta tubulin antibody (bs-4511R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥580 - 4680
- Bioss
- bs-4511R
- 2025年10月16日
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- 规格：
100ul/500ul/200ul/1000ul
| 规格： | 100ul | 产品价格： | ￥580.0 |
|---|---|---|---|
| 规格： | 500ul | 产品价格： | ￥2280.0 |
| 规格： | 200ul | 产品价格： | ￥980.0 |
| 规格： | 1000ul | 产品价格： | ￥4680.0 |
| 产品编号 | bs-4511R |
| 英文名称 | Beta tubulin Rabbit pAb, Loading Control |
| 中文名称 | 微管蛋白β tubulin/Tubulin β（内参）抗体 |
| 英文别名 | beta 4 tubulin; Tubulin-beta; Tubulin beta; beta 5 tubulin; betaTubulin; beta-Tubulin; dJ40E16.7; TUBB; TUBB2; TUBB2A; TUBB5; tubulin beta 2A; Tubulin beta chain; Tubulin beta-5 chain; TUBB4A; TUBB4; Tubulin 5 beta; Tubulin beta-4 chain; TBB4A_HUMAN; Tubulin beta-4A chain. |
| 产品应用 | WB=1:10000-100000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat, 肾, 肾, 肾, 肾, 肾, 肾, 肾, 肾, 肾, 肾 |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human tubulin Beta |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 50 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microtubules > Tubulin Tags & Cell Markers > Subcellular Markers > Cytoskeleton > Microtubules |
| 亚基 | Dimer of alpha and beta chains. May interact with RNABP10. Interacts with PIFO. Interacts with MX1. |
| 亚细胞定位 | Cytoplasmic, cytoskeleton. |
| 组织特异性 | Ubiquitously expressed with highest levels in spleen, thymus and immature brain. |
| 翻译后修饰 | Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules (Probable). |
| 相似性 | Belongs to the tubulin family. |
| 功能 | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:10000-100000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1ug/Test} |
Sample:
Lane 1,3,5: Mouse Cerebellum tissue lysates
Lane 2,4,6: Rat Cerebellum tissue lysates
Primary: Anti-Beta tubulin (bs-4511R) at 1/10000~1/50000 dilution
Secondary: HRP conjugated Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 48 kDa
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beta tubulin Polyclonal Antibody, Unconjugated (bs-4511R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beta tubulin Polyclonal Antibody, Unconjugated (bs-4511R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beta tubulin Polyclonal Antibody, Unconjugated (bs-4511R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beta tubulin Polyclonal Antibody, Unconjugated (bs-4511R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
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文献和实验该产品被引用文献
[IF={{ 8.5 }}] {Genghua Chen. et al. Bulk and single-cell alternative splicing analyses reveal roles of TRA2B in myogenic differentiation. CELL PROLIFERAT. 2023 Sep;:e13545} {WB} {Chicken}
[IF={{ 8.2 }}] {Wen Wang. et al. Characterization of a novel γ-type carbonic anhydrase, Sjγ-CA2, in Saccharina japonica: Insights into carbon concentration mechanism in macroalgae. INT J BIOL MACROMOL. 2024 Apr;263:130506} {WB} {Saccharina japonica}
[IF={{ 7.109 }}] {Wu Yutong. et al. Reduced osteoclast-derived apoptotic bodies in bone marrow characterizes the pathological progression of osteoporosis. CELL DEATH DISCOV. 2023 Apr;9(1):1-9} {Other} {}
[IF={{ 7.051 }}] {Zhao, Binggong. et al. USP13 promotes breast cancer metastasis through FBXL14-induced Twist1 ubiquitination. CELL ONCOL. 2023 Feb;:1-17} {WB} {Human}
[IF={{ 6.583 }}] {Wu MM et al. Lovastatin attenuates renal tubule‐specific ABCA1 knockout‐induced hypertension by inhibiting the epithelial sodium channel. Br J Pharmacol. 2019 Jun 21.} {WB} {Mouse}
相关实验
duplex PCR amplification, which is the coamplification of a fragment from the region of interest and a control fragment (e.g., the actin gene, or the tubulin gene). This approach allows for estimating relative levels of specific histone modifications
Methods to Detect Protein Glutathionylation
1 M DTT 4× nonreducing SDS loading buffer Anti‐GAPDH rabbit polyclonal antibody (or primary antibody
Studies of the Ubiquitin Proteasome System
Assays of E1 and E2 Activity Basic Protocol 1: Thiolester Formation Between Rabbit E1 and Ubiquitin Basic Protocol 2: Thiolester Formation Between E2 and Ubiquitin Binding of Ubiquitin‐Proteasome Proteins Basic Protocol 3: Binding of E2s to E3s Alternate
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Beta tubulin Rabbit pAb, Loading Control(bs-4511R)-100ul/500ul/200ul/1000ul
￥580 - 4680
