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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-30 ~ -15℃保存。-20 ~ 0℃运输。
- 英文名:
Single Cell Sequence Specific Amplification Kit
- 库存:
充足
- 供应商:
诺唯赞
- 规格:
200 rxns
Single Cell Sequence Specific Amplification Kit
用于单细胞或微量总RNA的预扩增试剂盒

单细胞或微量总RNA中转录组扩增;2 - 1,000个细胞的一步扩增

· 适用于1-1000个细胞
· 一步法RT-PCR预扩增
· 可扩增500个靶基因
· 扩增产物适合用任何实时荧光定量PCR仪分析
· 高灵敏度,低成本

Single Cell Squence Specific Amplification Kit 是基于一步RT-PCR的扩增方法,实现单细胞或者微量总RNA中转录组的扩增,检测单个细胞之间不同基因的表达水平。本试剂盒实现了细胞裂解、RNA逆转录和PCR反应在同一管内完成,不需要额外的移管操作,具有节约时间、减少实验误差、降低污染、提高灵敏度等优势。



-30 ~ -15℃保存。-20 ~ 0℃运输。
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文献和实验Single-Cell cDNA Library Construction Using Cycling aRNA Amplification
to maintain the completeness of a cDNA library, resulting in a loss of rare cDNAs. However, gene expression analysis of specific cell populations within a heterogeneous tissue is essential for research in vivo, requiring a method to generate cDNA libraries
Single-Cell Laser-Capture Microdissection and RNA Amplification
Generating gene-expression profiles from laser-captured cells requires the successful combination of laser-capture microdissection, RNA extraction, RNA amplification, and microarray analysis. To permit single-cell gene-expression profiling
Nucleic Acid Sequence-Based Amplification
Nucleic acid sequence-based amplification (NASBA; bioM�rieux, Boxtel, The Netherlands) is a commercially available amplification procedure that uses RNA as the target. It makes use of the simultaneous enzymatic activities of avian myeloblastosis
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