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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
qPCR Lentivirus Titration(Titer) Kit
- 库存：
慢病毒滴度检测试剂盒
- 供应商：
研卉生物
- 规格：
100Reactions
qPCR Lentivirus Titration(Titer) Kit，慢病毒滴度检测试剂盒ABM公司
货号：LV900
The Lentivirus Titration Kit
provides a fast and simple method for titrating lentivirus. This kit employs a quick RNA extraction step and determines viral RNA using qRT-PCR. The whole assay could be completed in only 2 hours. The titer primers supplied in this kit are designed for all HIV-1 based vectors detection. With the help of the on-line titer calculator, titration of any lentiviral preparation could be easier than ever before.
货号：LV900
The Lentivirus Titration Kit
provides a fast and simple method for titrating lentivirus. This kit employs a quick RNA extraction step and determines viral RNA using qRT-PCR. The whole assay could be completed in only 2 hours. The titer primers supplied in this kit are designed for all HIV-1 based vectors detection. With the help of the on-line titer calculator, titration of any lentiviral preparation could be easier than ever before.
Advantages of ABM's Lentiviral qPCR Titer Kit
- no RNA purification -- saves time and eliminates inaccuracy
- ready-to-use reagent mix -- reduces variability
- qRT-PCR in one step -- more sensitive and accurate than other methods
- no NTC amplification -- our titration kit is the only one on the market that completely eliminates NTC signal due to our unique primer design. Similar kits from other companies all give NTC amplification, which compromise accuracy for viral titration
- qPCR Lentivirus Titration(Titer) Kit慢病毒滴度检测试剂盒参考文献：
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Product References
- Zhang, M et al. "Epigenetically mediated spontaneous reduction of NFAT1 expression causes imbalanced metabolic activities of articular chondrocytes in aged mice." Osteoarthritis and Cartilage 24.7:1274–1283 (2016). DOI: 10.1016/j.joca.2016.02.003. Application: Titer Determination.
- Robitaille, K et al. "High-throughput Functional Genomics Identifies Regulators of Primary Human Beta Cell Proliferation." The Journal of Biological Chemistry 291.9:4614-4625 (2016). DOI: 10.1074/jbc.M115.683912. Application: Titer Determination.
- Theis, et al. "Lentiviral Delivery of miR-133b Improves Functional Recovery After Spinal Cord Injury in Mice." Mol. Neurobiol. : (2016). DOI: 10.1007/s12035-016-0007-z. Application: Titer Determination.
- Henry, A et al. "New role of osteopontin in DNA repair and impact on human glioblastoma radiosensitivity." Oncotarget 2016:1-14 (2016). Application: Titer Determination.
- Margul, D et al. "Reducing neuroinflammation by delivery of IL-10 encoding lentivirus from multiple-channel bridges." Bioengineering and Translational Medicine 1.2:136-148 (2016). DOI: 10.1002/btm2.10018.
- Jin, C et al. "Safe engineering of CAR T cells for adoptive cell therapy of cancer using long-term episomal gene transfer." EMBO Mol. Med. 8.7:702-711 (2016). DOI: 10.15252/emmm.201505869. Application: Production and Titration of Vectors.
- Kurata, M et al. "Using genome-wide CRISPR library screening with library resistant DCK to find new sources of Ara-C drug resistance in AML." Sci. Rep. 6: (2016). DOI: 10.1038/srep36199. Application: Titer Determination.
- Robitaille, K et al. "High-throughput Functional Genomics Identifies Regulators of Primary Human Beta Cell Proliferation." J. Biol. Chem. 291(9):4614-4625 (2016). DOI: 10.1074/jbc.M115.683912 .
- Zhang, M et al. "Epigenetically mediated spontaneous reduction of NFAT1 expression causes imbalanced metabolic activities of articular chondrocytes in aged mice." Osteoarthritis Cartilage. 24(7):1274-1283 (2016). DOI: 10.1016/j.joca.2016.02.003.
- Kurata, M et al. "Using genome-wide CRISPR library screening with library resistant DCK to find new sources of Ara-C drug resistance in AML." Sci. Rep. 6:36199 (2016). DOI: doi:10.1038/srep36199.
- Margul, DJ et al. "Reducing neuroinflammation by delivery of IL-10 encoding lentivirus from multiple-channel bridges." Bioeng Transl Med. 1(2):136-148 (2016). DOI: DOI: 10.1002/btm2.10018.
- Theis, T et al. "Lentiviral Delivery of miR-133b Improves Functional Recovery After Spinal Cord Injury in Mice." Mol Neurobiol. : (2016). DOI: 10.1007/s12035-016-0007-z.
- Henry, A et al. "New role of osteopontin in DNA repair and impact on human glioblastoma radiosensitivity." Oncotarget 7(39):63708-63721 (2016). DOI: 10.18632/oncotarget.11483 .
- Jin, C et al. "Safe engineering of CAR T cells for adoptive cell therapy of cancer using long-term episomal gene transfer." EMBO Mol Med. 8(7):702-711 (2016). DOI: 10.15252/emmm.201505869.
- Mak, IW et al. "The epigenetic regulation of SOX9 by miR-145 in human chondrosarcoma." J Cell Biochem 116(1):37-44 (2015). DOI: 10.1002/jcb.24940. PubMed: 25145279.
- Sundararaman, B. et al. "Resources for the comprehensive discovery of functional RNA elements." bioRxiv beta: The Preprint Server for Biology : (2015). DOI: http://dx.doi.org/10.1101/030486.
- Thomas, A. M. et al. "Sponge-mediated lentivirus delivery to acute and chronic spinal cord injuries." J Control Release :1-10 (2015). DOI: 10.1016/j.jconrel.2015.02.032. PubMed: 25724274.
- Sheu, J. et al. "Large-scale production of lentiviral vector in a closed system hollow fiber bioreactor." Mol. Ther. Methods Clin. Dev. : (2015). DOI: doi:10.1038/mtm.2015.20.
- Fransson, M. et al. "Mesenchymal stromal cells support endothelial cell interactions in an intramuscular islet transplantation model." Regen Med Res. 1: (2015). DOI: 10.1186/s40340-015-0010-9.
- Cao, Z. et al. "Ubiquitin Ligase TRIM62 Regulates CARD9-Mediated Anti-fungal Immunity and Intestinal Inflammation." Immunity. 4:715-726 (2015). DOI: doi: 10.1016/j.immuni.2015.10.005. PubMed: 26488816.
- Krassnig, S. et al. "Influence of Lentiviral β-Synuclein Overexpression in the Hippocampus of a Transgenic Mouse Model of Alzheimer's Disease on Amyloid Precursor Protein Metabolism and Pathology." Neurodegener Dis. 4:243-257 (2015). DOI: 10.1159/000430952. PubMed: 26111745.
- Das, B. et al. "Short chain fatty acids potently induce latent HIV-1 in T-cells by activating P-TEFb and multiple histone modifications." Virology. :65-81 (2015). DOI: 10.1016/j.virol.2014.10.033. PubMed: 25463605.
- Shang, R. et al. "Ribozyme-enhanced single-stranded Ago2-processed interfering RNA triggers efficient gene silencing with fewer off-target effects." Nat. Commun. : (2015). DOI: doi:10.1038/ncomms9430.
- Gower, RM et al. "Modulation of leukocyte infiltration and phenotype in microporous tissue engineering scaffolds via vector induced IL-10 expression." Biomaterials 6 (35):2024-2031 (2014). DOI: 10.1016/j.biomaterials.2013.11.036. PubMed: 24309498. Application: Lentivirus Titer.
- Boehler, RM et al. "Lentivirus delivery of IL-10 to promote and sustain macrophage polarization towards an anti-inflammatory phenotype." Biotechnol. Bioeng. 111 (6):1210-1221 (2014). DOI: 10.1002/bit.25175. PubMed: 24375008. Application: Lentivirus Titer.
- Chen, YL et al. "Small interfering RNA targeting nerve growth factor alleviates allergic airway hyperresponsiveness." Mol Ther Nucleic Acids 3:e158 (2014). DOI: 10.1038/mtna.2014.11. PubMed: 24714423. Application: Lentivirus Titer.
- Thomas, AM et al. "Sonic hedgehog and neurotrophin-3 increase oligodendrocyte numbers and myelination after spinal cord injury." Royal society of chemistry : (2014). DOI: 10.1039/C4IB00009A. PubMed: 24873988. Application: Lentivirus Titer.
- George, R et al. "A SHORT INTERFERING RNA MOLECULAR BEACON FOR THE ATTENUATION OF MYCOBACTERIAL INFECTION." American Journal of Biochemistry and Biotechnology 10:40-49 (2014). DOI: 10.3844/ajbbsp.2014.40.49. Application: Viral Titer Determination.
- Yu, X et al. "Short-Chain Fatty Acids from Periodontal Pathogens Suppress Histone Deacetylases, EZH2, and SUV39H1 To Promote Kaposi's Sarcoma-Associated Herpesvirus Replication." J Virol. 88(8):4466-79 (2014). DOI: 10.1128/JVI.03326-13. PubMed: 24501407. Application: Viral Titer.
- Puppo, A et al. "Retinal transduction profiles by high-capacity viral vectors." Gene Ther. 21(10):855-65 (2014). DOI: 10.1038/gt.2014.57. PubMed: 24989814. Application: Lentivirus Titer.
- Chakraborty, S et al. "A CRISPR/Cas9-based system for reprogramming cell lineage specification." Stem Cell Reports 3(6):940-7 (2014). PubMed: 25448066.
- Taylor, HE et al. "The innate immune factor apolipoprotein L1 (APOL1) restricts HIV-1 infection." J of Virol. : (2013). PubMed: 24173214.. Application: Lentivirus Titer.
- Thomas, AM et al. "Polysaccharide-modified scaffolds for controlled lentivirus delivery in vitro and after spinal cord injury." J Control Release 170 (3):421-429 (2013). DOI: 10.1016/j.jconrel.2013.06.013. Application: Lentivirus Titer.
- Yu, X et al. "Short-chain fatty acids from periodontal pathogens suppress histone deacetylases, EZH2, and SUV39H1 to promote Kaposi's sarcoma-associated herpesvirus replication." J. Virol. 88 (8):4466-4479 (2014). DOI: 10.1128/JVI.03326-13. PubMed: 24501407. Application: Lentivirus Titer.
- Hbattacherjee, A et al. "Bone morphogenetic protein 4 mediates estrogen-regulated sensory axon plasticity in the adult female reproductive tract." J Neurosci 33 (3):1050-1061a (2013). DOI: 10.1523/JNEUROSCI.1704-12.2013. PubMed: 23325243. Application: Lenti Titer.
- Bhattacherjee, A et al. "Bone morphogenetic protein 4 mediates estrogen-regulated sensory axon plasticity in the adult female reproductive tract." J. Neurosci. 3 (33):1050-1061a (2013). DOI: 10.1523/JNEUROSCI.1704-12.2013. PubMed: 23325243. Application: Lentivirus Titer.
- Azarin, S. M. et al. "In vivo capture and label-free detection of early metastatic cells." Nat. Commun. : (2015). DOI: 10.1038/ncomms9094.
- Chahine, NO et al. "Effect of age and cytoskeletal elements on the indentation-dependent mechanical properties of chondrocytes." PLoS ONE 8 (4):e61651 (2013). DOI: 10.1371/journal.pone.0061651. PubMed: 23613892. Application: Lentivirus Titer.
- WZang, ZY et al. " Immortalized porcine liver sinusoidal endothelial cells: an in vitro model of xenotransplantation-induced thrombocytopenia." Xenotransplantation 19 (4):249-255 (2012). DOI: 10.1111/j.1399-3089.2012.00715.x. PMID: 22909138. Application: Lentivirus Titer.
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文献和实验相关实验
Titration of Lentiviral Vectors
5-10%) Titer is a number (percentage) of cells transduced by a given vol and counted on D2 e.g. 1� gives you 10% of positivie cells and you had 50.000 cells on D2, so you have 5.000TU/� --> 5x106 TU/ml Day 7-8 isolate DNA for QPCR
慢病毒转染肝细胞方法Lentivirus Transduction of Hematopoietic Cells
Lentivirus Transduction of Hematopoietic Cells Ming-Jie Li and John J. Rossi Division of Molecular Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010
Lentiviral Vector Mediated Transgenesis
one to perform a standard curve) Kit for preparing qPCR master mix (TaqMan universal PCR master mix, no AmpErase UNG; Applied Biosystems, cat. no. 4324020, including 2× reaction buffer
qPCR Lentivirus Titration(Titer) Kit，慢病毒滴度检测试剂盒ABM公司
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