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- 技术资料
- 保存条件:
低温运输,-20℃保存
- 保质期:
1年
- 英文名:
pDK101
- 库存:
大量
- 供应商:
信裕生物
质粒编号:
质粒名称: pDK101
其他名称:
质粒大小(bp): 3027
抗性:
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号:
其他属性:
| Notes: |
Deposited by: Bernard Weisblum Restriction digests of the clone give the following sizes (kb): BglI–1.7, 1.3; EcoRV–3.0; PstI–3.0. (ATCC staff) Vector useful for cloning-double stranded amplification products. Permits synthesis of ssDNA and contains promoters for in vitro RNA synthesis. [1] The plasmid contains the following restriction sites (bp from nt 1): BglI–1533, 2854; EcoRV–73; NcoI–37, 61; NdeI–105; NotI–85; PstI–93; PvuI–1786, 2885; PvuII–348, 2915; SacI–114; SalI–99; XcmI–44, 59; XmnI–2014. [1] The order of the major features in this plasmid is: T7/ApaI/MCS/NsiI/lacZ’SP6 – pMB1 ori – pMB1 – ampR – f1 ori. (personal communication) Medium is 1227 LB plus ampicillin. Hosts: E.coli, E.coli JM109.
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质粒图谱:
质粒序列:
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文献和实验Transformation of HB-101 Competent Cells
实验步骤 1. Thaw tubes of competent cells on ice, occasionally inverting gently to mix. 2. Pre-cool Falcon 2059 tubes on ice. 3. Aliquot 30 ul cells into each tube. 4. Add up to 10 ul DNA in TE or ligation
101-A数显鼓风电热恒温箱 术参数: 材质:工作室采用优质钢板。表面喷涂耐高温,耐腐蚀银浆 温度范围:50~300℃ 温度波动度:≤±1℃; 温度均匀度:≤±2.5﹪;; 型号 工作室尺寸 电压 功率
【资源】Methods In Molecular Biology Collection - Volumes 101-200
缺9卷 Methods In Molecular Biology Collection - Volumes 101-200 101-Mycobacteria Protocols-Methods in Mol Biol Vol 101.pdf 102-Bioluminescence Methods and Protocols-Methods in Mol Biol Vol 102.pdf 103-Pichia Protocols-Methods in Mol Biol Vol
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