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- EASYBIO
- BE3107-50
- 2025年12月13日
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- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
多克隆
- 抗体英文名：
Aquaporin 4 Mouse Monoclonal Antibody
- 规格：
支
Aquaporin 4 belongs to the Aquaporin family of integral membrane proteins that conduct water through the cell membrane.
Product Name:Aquaporin 4 Mouse Monoclonal Antibody
Clone Number: 5H2
Isotype: IgG1
Storage Buffer :PBS, pH 7.4, containing 0.02% sodium azideas Preservative and 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:1,000 IF: 1:100-200
Optimal dilutions should be determined by the end user.
Specificity: The Aquaporin 4 Mouse Monoclonal antibody detects endogenous Aquaporin 4 proteins.
Molecular Weight:48KD
Form: Liquid
Reactivity:H, M, R
Western blot analysis of 1) Hela, 2)Mouse Heart tissue ,3) Rat Heart Tissue with Aquaporin 4 mAb diluted at 1:2,000.
IF analysis of Hela with BE3107(Left) and DAPI (Right) diluted at 1:100.
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
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