- ￥400
- EASYBIO
- BE2067-10
- 2025年12月26日
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- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
多克隆
- 抗体英文名：
mCherry-Tag Mouse Mixture Monoclonal Antibody
- 规格：
10μl/支
mCherryis a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct with peak absorption/emission at 587 nm and 610 nm, respectively. It is resistant to photobleaching and is stable. mCherry is sometimes preferred to other fluorophores due to its colour, as well as its photostability compared to other monomeric fluorophores.
Product Name:mCherry Mouse Mixture Monoclonal Antibody
Isotype: IgG1
Storage Buffer :PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:5,000 IP: 1:200
Optimal dilutions should be determined by the end user.
Specificity: The mCherry tag antibody can recognize mCherry and mCherry tag fusion proteins.
Form: Liquid
Reactivity:ALL
Western blot analysis of mCherry recombinant protein with BE2067 diluted at
1) 1:5,000
2) 1:10,000
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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