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- 2025年07月15日
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- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
多克隆
- 抗体英文名：
Cytokeratin 18 Mouse Monoclonal Antibody
- 规格：
100μl/支
Keratin 18 is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.Keratin 18 is often used together with keratin 8 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.
Product Name:Cytokeratin 18 Mouse Monoclonal Antibody
Clone Number: 3F8
Isotype: IgG1
Storage Buffer :PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:500-1,000
Optimal dilutions should be determined by the end user.
Specificity: Antibody can detects endogenous Cytokeratin 18 protein.
Alternative Names: Cell proliferation inducing gene 46 protein, CK 18, CYK 18, K 18, Keratin 18, Ketatin D, KRT18
Molecular Weight:46KD
Form: Liquid
Reactivity:H, M
Western blot analysis of 1) Hela, 2) HepG2, 3) Mouse Skeletal Muscle tissue with Cytokeratin 18 Mouse MAb (3F8) diluted at 1:1,000..
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
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