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- EASYBIO
- BE3107-50
- 2025年12月13日
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- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
多克隆
- 抗体英文名：
Aquaporin 4 Mouse Monoclonal Antibody
- 规格：
支
Aquaporin 4 belongs to the Aquaporin family of integral membrane proteins that conduct water through the cell membrane.
Product Name:Aquaporin 4 Mouse Monoclonal Antibody
Clone Number: 5H2
Isotype: IgG1
Storage Buffer :PBS, pH 7.4, containing 0.02% sodium azideas Preservative and 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:1,000 IF: 1:100-200
Optimal dilutions should be determined by the end user.
Specificity: The Aquaporin 4 Mouse Monoclonal antibody detects endogenous Aquaporin 4 proteins.
Molecular Weight:48KD
Form: Liquid
Reactivity:H, M, R
Western blot analysis of 1) Hela, 2)Mouse Heart tissue ,3) Rat Heart Tissue with Aquaporin 4 mAb diluted at 1:2,000.
IF analysis of Hela with BE3107(Left) and DAPI (Right) diluted at 1:100.
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文献和实验Monoclonal Antibody Production
Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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