Maltose binding protein (MBP) is a useful affinity tag that can increase the expression level and solubility of the MBP tagged protein. It promotes proper folding of the fusion protein, and it can be also used to prevent an insoluble form (inclusion bodies). MBP Tag antibody is suitable for detecting fusion proteins that contain a MBP-tag.
Product Name:MBP-tag Rabbit polyclonal antibody
Immunogen: Full-length MBP protein
Purification: MBP protein affinity purification
Tested applications: WB IP
Recommended dilutions: WB: 1:2000-5000 IP:1:200
Optimal dilutions/concentrations should be determined by the end user.
Form:Liquid
Concentration:1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
Storage: MBPantibody is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Multi Tag Recombinant protein (expressed in E.coli)
Primary antibody Anti-MBP PAB (BE2021) lane 1: 1:2000 lane 2 1:5000
Secondary antibody Goat-anti Rabbit IgG (H&L) HRP( BE0101)1:10000