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- EASYBIO
- BE3139-50
- 2025年12月14日
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
多克隆
- 抗体英文名：
ErbB-2/HER-2 Mouse Monoclonal Antibody
- 规格：
支
ErbB 2 is a receptor tyrosine kinase of the ErbB 2 family. It is closely related instructure to the epidermal growth factor receptor. ErbB 2 oncoprotein is detectable in a proportion of breast and other adenocarconomas, as well as transitional cell carcinomas. In the case of breast cancer, expression determined by immunohistochemistry has been shown to be associated with poor prognosis.
Product Name:ErbB-2/HER-2 Mouse Monoclonal Antibody
Clone Number: 1H8
Isotype: IgG1
Storage Buffer :PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:2,000-4,000 IHC: 1:200
Optimal dilutions should be determined by the end user.
Specificity: The ErbB-2/HER-2 Mouse Monoclonal antibody detects endogenous ErbB-2/HER-2 proteins.
Molecular Weight:138KD
Form: Liquid
Reactivity:H, R, M
Western blot analysis of 1) Hela, 2)Mouse Brain with ErbB-2/HER-2 mAb diluted at 1:4,000.
IHC staining of human breast cancer tissue with ErbB-2/HER-2 mouse mAb(1H8) diluted at 1:200.
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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