c-Myc-tag antibody is part of the Tag series of antibodies, the best quality in the research. The immunogen of c-Myc tag antibody is a synthetic peptide corresponding to residues 410-419 of the human p62 c-myc protein conjugated to KLH. C-Myc tag antibody is suitable for detecting the expression level of c-Myc or its fusion proteins where the c-Myc tag is terminal or internal.
Product Name:c-Myc-tag mouse monoclonal antibody
Clone Number: 4E9
Isotype: IgG1
Immunogen: A synthetic peptide EQKLISEEDLcoupled to KLH.
Tested applications and dilutions:WB 1:5000-2000 IP 1:200 IF 1:2000
Produce anti- c-myc antibody affinity column purify c-myc recombinant proteins
Form:Liquid
Concentration: 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
Storage: c-Myc antibody is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
A: HEK 293 cell lysate without transfected
B: HEK 293 cell lysate transfected with c-myc-tag protein
C: IP (PBS + anti- c-myc mAb 4℃overnight)
D: IP (HEK293 transfected+protein G+anti- c-myc mAb 4℃overnight)
E: IP (HEK293 transfected+RIPA+Protein G 4℃overnight)
F: Multi Tag Recombinant protein (expressed in E.coli)
TP=Target Protein HC=Heavy chain LC=Light Chain
IF analysis of 293T cells transfected with a MYC-tagged protein, using easybio MYC-Tag (4E9) Mouse mAb at a 1:2000 dilution (blue DAPI ,red anti-C-MYC)