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- 2025年12月28日
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- 技术资料
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
多克隆
- 抗体英文名：
HA-Tag Mouse Monoclonal Antibody
- 规格：
10μl/支
HA tag Mouse mAb is part of the series of Tag antibodies, the excellent quality in the research. HA tag antibody is a highly sensitive and affinity monoclonal antibody applicable to HA-tagged fusion protein detection. HA tag antibody can detect HA tags in internal, C-terminal, or N-terminal recombinant proteins.
Product Name:HA-tag mouse monoclonal antibody
Clone Number: 2B11
Isotype: IgG1
Immunogen: A synthetic peptide YPYDVPDYAcoupled to KLH.
Tested applications and dilutions: WB 1:5000-2000 IP 1:200 IF 1:1000
Produce anti- HA antibody affinity column purify HA recombinant proteins
Form:Liquid
Concentration: 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
Storage: HAantibody is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
A: HEK 293 cell lysate without transfected
B: HEK 293 cell lysate transfected with HA-tag protein
C: IP (HEK 293 cell without transfected + anti- HA mAb 4℃overnight)
D: IP (PBS + anti- HA mAb 4℃overnight)
E: IP (HEK293 transfected+protein G+anti- HA mAb 4℃overnight)
F: IP (HEK293 transfected+RIPA+Protein G 4℃overnight)
G: Multi Tag Recombinant protein (expressed in E.coli)
TP=Target Protein HC=Heavy chain LC=Light Chain
IF analysis of 293T cells transfected with a HA-tagged protein, using easybio HA-Tag (2B11) Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-HA)
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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